Plants infected with cucumber mosaic virus (CMV) (KIN strain) produce a mild mosaic disease on tobacco whereas infections of CMV with satellite RNA (strain Y) cause a severe yellow mosaic. Analysis of recombinant and mutant forms of satellite RNA identified a site (nucleotides 185/186) in the Y satellite RNA that affects the ability to induce the yellow mosaic in combination with CMV but not wi...

AtMPB2C is the Arabidopsis (Arabidopsis thaliana) homolog of MPB2C, a microtubule-associated host factor of tobacco mosaic virus movement protein that was been previously identified in Nicotiana tabacum. To analyze the endogenous function of AtMPB2C and its role in viral infections, transgenic Arabidopsis plant lines stably overexpressing green fluorescent protein (GFP)-AtMPB2C were established...

The strategy for mass cloning of plants by micro­ propagation depends on disease-indexing the donor plant, and eliminating any diseases found, before setting up the explant or meristem cultures. Details of tissue culture media have been published for most commercially important species [1]. Fungal, bacterial and mycoplasmal pathogens can be successfully elim­ inated by the addition of the appro...

Minthostachys mollis (Kunth.) Griseb., “peperina”, a member of the family Lamiaceae, is an aromatic species used in modern pharmacology and medicine. It is widely distributed in the Andes, from Venezuela and Colombia to Argentina. In Argentina, the main exploitation area of peperina includes the mountain area of the province of Córdoba, where the plant is indiscriminately harvested, leading to ...

The N gene conditions for resistance to Tobacco mosaic virus (TMV) but only below 28 degrees C. However, a TMV-based vector expressing green fluorescent protein (TMV-GFP) showed only limited movement at 33 degrees C in tobacco plants harboring the N gene and other genes cointrogressed from Nicotiana glutinosa. TMV-GFP moved efficiently in tobacco plants that either lacked these genes or that co...

Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS...

In transgenic Arabidopsis (Arabidopsis thaliana), expression of the Cucumber mosaic virus (CMV) 2b silencing suppressor protein from the severe subgroup IA strain Fny disrupted microRNA (miRNA)-regulated development but orthologs from mild subgroup II strains (Q and LS) did not, explaining strain-specific differences in symptom severity. However, it is unknown which miRNAs affected by Fny2b cri...

A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into ...