BACKGROUND Although cisplatin is extensively used in the clinical field, its intrinsic toxicity limits its clinical use. We investigated nanoparticle formations of poly(acrylic acid-co-methyl methacrylate) (PAA-MMA) incorporating cisplatin and their antitumor activity in vitro and in vivo. METHODS Cisplatin-incorporated nanoparticles were prepared through the ion-complex formation between acr...

Background Colon cancer (CRC) is the second leading cause of cancer-related deaths in US. CRC incidence on rise and there an alarming increase young onset cases. Immune checkpoint inhibitors (ICIs) have yielded promising anti-tumor results microsatellite instable high (MSI-high) patients, which represent only 15% tumors. The remaining 85% are denoted as stable (MSS) unresponsive to ICI. Using a...

ABSTRACT APS001F is a strain of Bifidobacterium longum genetically engineered to express cytosine deaminase that converts 5-fluorocytosine (5-FC) 5-fluorouracil. In the present study, antitumor effects plus 5-FC (APS001F/5-FC) in combination with anti-PD-1 monoclonal antibody were investigated using CT26 syngeneic mouse model. Both dosing APS001F/5-FC before and after mAb exhibited as well prol...

Cancers can be addicted to continued and relatively high expression of nuclear oncoproteins. This is evident in colorectal cancer (CRC) where the oncoprotein and transcription factor MYB is over expressed and essential to continued proliferation and tumour cell survival. Historically, targeting transcription factors in the context of cancer has been very challenging. Nevertheless, we formulated...

Despite the impressive rates of clinical response to programmed death 1 (PD-1) blockade in multiple cancers, the majority of patients still fail to respond to this therapy. The CT26 tumor in mice showed similar heterogeneity, with most tumors unaffected by anti-PD-1. As in humans, response of CT26 to anti-PD-1 correlated with increased T- and B-cell infiltration and IFN expression. We show that...

BACKGROUND Resistance to chemotherapy is a growing concern, thus natural anticancer agents are drawing the attention of many scientists and clinicians. One natural anticancer agent, umbelliprenin, is a coumarin produced by many species of Ferula. OBJECTIVES We aimed to examine the inhibitory effect of umbelliprenin on human and mouse bone marrow-derived stem cells (BMDSCs), peripheral blood m...

The major stress-inducible heat shock protein 70 (Hsp70) is frequently present on the cell surface of human tumours, but not on normal cells. Herein, the binding characteristics of the cmHsp70.1 mouse monoclonal antibody (mAb) were evaluated in vitro and in a syngeneic tumour mouse model. More than 50% of the CT26 mouse colon carcinoma cells express Hsp70 on their cell surface at 4°C. After a t...

Background Immunotherapy, including adoptive cell therapy (ACT) and immune checkpoint inhibitors (ICIs), has a limited effect in most patients with colorectal cancer (CRC), the efficacy is further liver metastasis. Lack of antitumor lymphocyte infiltration could be major cause, there remains an urgent need for more potent safer therapies CRC. Methods In this study, antitumoral synergism low mol...

Trong nghiên cứu này, thành phần hóa học của cao chiết ethanol từ rễ loài W. florida “Pink Poppet” đã được đánh giá qua các phản ứng định tính. Kết quả cho thấy, trong có flavonoid, terpenoid, coumarin và saponin triterpene, nhưng không alkaloid, steroid glycoside tim. Cao xác hoạt tính kháng oxy thông khả năng loại bỏ gốc tự do DPPH với trị EC50 = 81,13 µg/mL. Nghiên sau đó gây độc trên dòng t...

Rationale: Cisplatin (CDDP) is a broad-spectrum anticancer drug but chemoresistance to CDDP impedes its wide use for cancer therapy. Autophagy is an event occurring in the cytoplasm and cytoplasmic LC3 puncta formation is a hallmark of autophagy. Graphene oxide (GO) is a nanomaterial that provokes autophagy in CT26 colon cancer cells and confers antitumor effects. Here we aimed to evaluate whet...