Preparations of killed Corynebacterium parvum were injected into 14-day-old growing mammary adenocarcinoma cell tumors in DBA/2 mice. The tumors and the microscopic métastasesundergo rapid and lasting rejection and the animals are protected against challenges of large quantities of tumor cells of the same line. This protection can be overcome if the challenge dose is massive. Groups of tumor-b...

BACKGROUND Cryptosporidium parvum (C. parvum) is a tiny protozoal parasite that can cause gastrointestinal illness in a wide variety of mammals, including humans, cattle, sheep, goats, pigs, and horses. It also occurs in various wildlife species such as deer, raccoons, opossums, and rabbits (Fayer et al. and Ungar 1990). In cattle, clinical disease and shedding of the parasite is usually limite...

OBJECTIVES This study aims to develop a high-sensitivity antibody diagnostic kit that will enable a rapid and accurate detection of Cryptospofidium parvum and Giardia lamblia in patients with diarrhea. METHODS The cultivated C. parvum oocysts and G. lamblia cysts in each calf and dog were injected to mice to obtain antibodies, which were titrated. Spleen cells of the immunized mouse were sepa...

Parasites from the Cryptosporidium genus are the most common cause of waterborne disease around the world. Successful management and prevention of this emerging disease requires knowledge of the diversity of species causing human disease and their zoonotic sources. This study employed a spatiotemporal approach to investigate sporadic human cryptosporidiosis in New South Wales, Australia, betwee...

BACKGROUND Ureaplasma urealyticum and U. parvum have been associated with respiratory diseases in premature newborns, but their role in the pathogenesis of the respiratory distress syndrome (RDS) is unclear. The aim of this study was to detect, using molecular techniques, the role of Mycoplasma spp. and Ureaplasma spp. in respiratory secretion and blood specimens of preterm newborns with or wit...

OBJECTIVE To determine the prevalence of Cryptosporidium parvum in diarrhoeal patients. DESIGN This was a laboratory-based cross sectional study on cryptosporidiosis in diarrhoeal patients. SETTING Department of Medical Laboratory Sciences, College of Health Sciences in Harare, Zimbabwe. SUBJECTS People of all ages with diarrhoea presenting at primary level health centres in Harare. MAI...

BACKGROUND Susceptibility or resistance to infection with Cryptosporidium parvum (C.parvum) correlates with Selenium (Se) deficiency in response to infection. Both adult Se-adequate and Se-deficient mouse models of cryptosporidiosis were used to study the cell-mediated immune response during the course of C. parvum infection. METHODOLOGY/PRINCIPAL FINDINGS Blood samples from mouse models were...

Populations of feral pigs (Sus scrofa) may serve as an environmental reservoir of Cryptosporidium parvum oocysts and Giardia sp. cysts for source water. We conducted a cross-sectional study to determine the prevalence of and associated demographic and environmental risk factors for the shedding of C. parvum oocysts and Giardia sp. cysts. Feral pigs were either live-trapped or dispatched from 10...

Healthy adults are susceptible to infection with small numbers of Cryptosporidium parvum oocysts, resulting in self-limited infection. We investigated if infection of humans with C. parvum is protective 1 year after primary exposure. At 1 year after a primary challenge with 30 to 10(6) oocysts, 19 healthy immunocompetent adults were rechallenged with 500 oocysts and monitored for the developmen...

Heat-killed, preservatIve-free preparations of strain CN 6134 Cosynebacterlum parvum have been demonstrated to Induceinterferonin culturesof normaladufthuman1lymphocytes.C. parvumwas also shownto inducehuman T-lymphocytes to proliferate In vitro. The maximum inter feron and proliferative response was observed 7 days after initiationof cultureat a finalC. parvumconcentration of 140 @&g/mb. Human...