UNLABELLED While cocoa plants regenerated from cryopreserved somatic embryos can demonstrate high levels of phenotypic variability, little is known about the sources of the observed variability. Previous studies have shown that the encapsulation-dehydration cryopreservation methodology imposes no significant extra mutational load since embryos carrying high levels of genetic variability are sel...

Common carp [Cyprinus carpio (C. carpio)] is an economically important fish species cultured mostly in Asia and Europe. Global production of farmed common carp was about 6.14% (3 172 488 tonnes) of the total world aquaculture production[1]. Artificial insemination can be applied to increase the production to meet this demand due to exploitation of natural stocks. Cryopreservation is a valuable ...

Objectives: The purpose of this study was to determine the appropriate cryopreservation period of tracheal allografts based on morphological and immunological ®ndings and to test the possibility of tracheal transplantation in rats using cryopreserved allografts without immunosuppression. Methods: Morphological and immunological studies were performed to compare the differences between non-cryop...

OBJECTIVE To review sperm cryopreservation usage rates, corresponding reproductive outcomes, and the current situation in our locality. DESIGN Retrospective case series. SETTING Assisted Reproductive Technology Unit of the Department of Obstetrics and Gynaecology, Prince of Wales Hospital and the Chinese University of Hong Kong. PARTICIPANTS There were 130 Chinese male patients who underw...

Pig embryos suffer severe sensitivity to hypothermic conditions, which limits their ability to withstand conventional cryopreservation. Research has focused on high lipid content of pig embryos and its role in hypothermic sensitivity, while little research has been conducted on structural damage. Documenting cytoskeletal disruption provides information on embryonic sensitivity and cellular resp...

In the last decades significant advances have been made in successful cryopreservation of mammalian oocytes. Human oocyte cryopreservation has practical application in preserving fertility for individuals at risk of compromised egg quality due to cancer treatments or advanced maternal age. While oocyte cryopreservation success has increased over time, there is still room for improvement. Oocyte...

Oocyte cryopreservation has great promise for applications in oocyte donation and fertility preservation and for decreasing the number of unused cryopreserved embryos. The Practice Committee has and will continue to review the published medical literature relating to oocyte cryopreservation at regular intervals and is prepared to reconsider its position when evidence warrants.

the majority of cancer treatments are invasive. gonadal injuries cause reductions in fertility which results in lack of hope for conception in cancer patients and frustration for their partners. fortunately, current advancements in cryopreservation and transplantation sciences regarding fertility preservation lead to cryostorage of gonads and preservation prior to the onset of chemo- and radiot...

oocyte, embryo and ovarian tissue cryopreservation are being increasingly proposed for fertility preservation among cancer patients undergoing therapy to enable them to have babies after the cancer is cured. embryo cryopreservation is not appropriate for single girls without any sperm partner and also because oocyte retrieval is an extended procedure, it is impossible in cases requiring immedia...