1 This article was supported by the Florida Agricultural Experimental Station. 2 Correspondence Karen Moore, Department of Animal Sciences, University of Florida, PO Box 110910, Gainesville, Florida 326110910, USA. E-mail: [email protected] Abstract Moore K, Bonilla AQ. Cryopreservation of Mammalian Embryos: the State of the Art. ARBS Annu Rev Biomed Sci 2006;8:19-32. Embryo cryopreservatio...

BACKGROUND We designed this study to detect the cryoinjury rate on human sperm after serial freezing and thawing, taking into consideration the effects of using cryovials and straws. MATERIALS AND METHODS In this experimental study, semen specimens obtained from 15 subjects were divided into normozoospermic and oligozoospermic groups. Each of the normozoospermic and oligozoo spermic semen spe...

Characteristically, uterine myometrial cells (MCs) are proliferative, inducing angiogenesis within the female reproductive organ. We evaluated whether MCs implanted into myocardium could also induce angiogenesis and restore heart function after injury. MCs were isolated from the adult rat uterus and cultured for three studies: 1) Intracellular VEGF levels were measured in MCs cultured with prog...

Successful long-term preservation of Mycobacterium tuberculosis cells is important for sample transport, research, biobanking, and the development of new drugs, vaccines, biomarkers, and diagnostics. In this report, Mycobacterium bovis bacillus Calmette-Guérin and M. tuberculosis H37Ra were used as models of M. tuberculosis complex strains to study cryopreservation of M. tuberculosis complex ce...

Cell features that may favor resistance to cryoinjury were investigated. Subpopulations of all tissue bank deposits tested (5 human cell lines and 19 mouse tumors) survived up to 16 years of storage at —78°. The replicating capacity of trypan blue-excluding cells after prolonged cryopreservation was estimated by comparing the number of unstained cells needed to produce < 100% lethal takes wi...

We studied microvascular injury after myocardial cryothermia in rats using intravital fluorescence microscopic techniques. Cryolesions were induced to the right ventricle by freezing with -160 degrees C (probe diameter: 5 mm) for a total of 5 min. Fluorescence microscopy was performed at 15, 30, 60, 90, and 120 min as well as at 3 and 7 days after cryothermia. Analysis of the epicardial microva...

PURPOSE Programmed Death-1 (PD-1) and its ligand, PD-L1, are regulators of immune/ inflammatory mechanisms. We explored the potential involvement of PD-1/PD-L1 pathway in the inflammatory response and tissue damage in cardiac injury models. EXPERIMENTAL DESIGN Ischemic-reperfused and cryoinjured hearts were processed for flow cytometry and immunohistochemical studies for determination of card...

UNLABELLED BACKGROUND The combination of myocardial cell transplantation and angiogenic gene transfer may improve postinfarction left ventricular (LV) perfusion. We evaluated the angiogenic effect of heart cells transfected with vascular endothelial growth factor (VEGF) and transplanted into a myocardial scar. METHODS AND RESULTS Donor rat heart cells were transfected with plasmids encoding V...

Stem cell therapy for myocardial infarction (MI) has been shown to improve cardiac function and reduce infarct size. Exercise training, in the form of cardiac rehabilitation, is an essential part of patient care post-MI. Hence, we tested the effects of acute and chronic aerobic exercise on stem cell retention and cardiac remodeling post-MI. Small epicardial MI's were induced in 12-month-old C57...

OBJECTIVE The objectives of this study were to analyze efficacy of immature and mature mouse oocytes after vitrification and warming by applying various combinations of cryoprotectants (CPAs) and/or super-rapid cooling using slush nitrogen (SN(2)). METHODS Four-week old ICR female mice were superovulated for GV- and MII-stage oocytes. Experimental groups were divided into two groups. Ethylene...