Azoreductases have been characterized as enzymes that can decolorize azo dyes by reducing azo groups. In this study, genes encoding proteins having homology with the azoreductase gene of Bacillus sp. OY1-2 were obtained from Bacillus subtilis ATCC6633, B. subtilis ISW1214, and Geobacillus stearotherophilus IFO13737 by polymerase chain reaction. All three genes encoded proteins with 174 amino ac...

Motility of the alkalophilic Bacillus sp. C-125, a flagellate bacterium, was demonstrated to be Na(+)- and pH-dependent. Flagellin protein from this strain was purified to homogeneity and the N-terminal sequence determined. Using the hag gene of Bacillus subtilis as a probe, the hag gene of Bacillus sp. C-125 was identified and cloned into Escherichia coli. Sequencing of this hag gene revealed ...

The primary habitat for Bacillus sp. is the soil. In this environment, they remain inactive until a suitable substrate (food source) becomes available. Usually these substrates include large molecules from the remains of plants and animals. The large molecules, like proteins, lipids, and carbohydrates are suitable substrate for Bacillus sp. because these strains excrete enzymes which break thes...

A strain of bacterium producing antifungal antibiotic was isolated and identification of the strain was attempted. We could identify the bacterium as being a Bacillus sp., based on morphological observation, physiological characteristics, and 16S rDNA sequence analysis, thus leading us to designate the strain as Bacillus sp. AH-E-1. The strain showed potent antibiotic activity against phytopath...

Metabolism of the phenylurea herbicide isoproturon by Sphingomonas sp. strain SRS2 was significantly enhanced when the strain was grown in coculture with a soil bacterium (designated strain SRS1). Both members of this consortium were isolated from a highly enriched isoproturon-degrading culture derived from an agricultural soil previously treated regularly with the herbicide. Based on analysis ...

A bacterial strain (strain PS) was isolated from the textile effluents carrying Serilene Black BNFS® (C.I. Disp. Blk. Mix) disperse dye. The isolate was able to decolorize the dye without the need for any exogenous carbon source. Full sequencing of its 16S rRNA indicated that Bacillus sp strain PS is related to Bacillus cereus groups.  Silica- gel-thin layer chromatography of Ser...

A chitosanase produced constitutively by Bacillus sp. MET 1299 was purified by SP-Sephadex column chromatography. The molecular weight was estimated to be 52 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Optimal enzyme activity was observed at a pH of 5.5 and temperature of 60 degrees C. The purified chitosanase showed high activity on 90% deacetylated colloidal c...

In this study we examined marine bacteria associated with different corals (Porites lutea, Galaxea fascicularis, Acropora sp. and Pavona sp.) collected from vicinity of Panjang island, Jepara, North Java Sea, Indonesia for their antimicrobial activities against the bacteria Echerichia coli, Bacillus subtilis, Staphylococcus lentus and the yeast Candida glabrata. A total of 13 bacterial isolates...

Textile effluent is one of the known sources of pollution, which usually contain several chemicals, which contaminate the receiving water. This study was conducted to understand the ability of Bacillus sp., and Pseudomonas sp., on decolourization and normalization of other physicochemical parameters, such as odour, temperature, pH, total solids, total dissolved solids, total suspended solids, t...