A comparison of Arabidopsis DNA sequences revealed that the final nucleotides at the 3' end of approximately half of the Arabidopsis mRNAs, immediately upstream of the poly(A) tail, differ from the corresponding genomic sequences. This suggests that extra nucleotides were added to these mRNAs at their 3' termini prior to polyadenylation. Among the mRNAs containing additional nucleotides, approx...

Recently, systems for rapid diagnosis of diseases and infections have motivated the development new DNA detection platforms. However, these platforms failed to reach market mainly because potential risk misdiagnosis. Thus, developing a system with an effective internal control, guaranteeing veracity result, could help overcome this barrier. The possibility regenerate microfluidic biosensing wou...

The bacterial recombinase RecA forms a nucleic acid-protein filament on single-stranded (ss) DNA during the repair of double-strand breaks (DSBs) that efficiently undergoes a homology search and engages in pairing with the complementary DNA sequence. We utilized the pairing activity of RecA-DNA filaments to tether biochemical activities to specific chromosomal sites. Different filaments with ch...

We report the replication technology of DNA chip using by sequence specific localization of nucleic acids via hybridization and electric transfer of the nucleic acids onto a new substrate without losing their array information. The denatured DNA fragments are first spotted and UV-cross-linked on a nylon membrane. The membrane is then immersed and hybridized in a DNA mixture solution that contai...

Tricyclo-DNA belongs to the family of conformationally restricted oligodeoxynucleotide analogues. It differs structurally from DNA by an additional ethylene bridge between the centers C(3') and C(5') of the nucleosides, to which a cyclopropane unit is fused for further enhancement of structural rigidity. The synthesis of the hitherto unknown tricyclodeoxynucleosides containing the bases cytosin...

Transitional cell carcinoma(T.C.C) of bladder display an upredictable biological behavior.Morphologic methods of grading this tumor are insuficient to predict the clinical outcome of the patients.the aim of this study was to investigate the relationships between histological grading staging and DAN ploidy especially in intermediate grade II(IIa and IIb). in a retrospective study  of tissue spec...

We successfully demonstrate a porous silicon (PS) double Bragg mirror by electrochemical etching at room temperature as a deoxyribonucleic acid (DNA) label-free biosensor for detecting ammonia-oxidizing bacteria (AOB). Compared to various other one-dimension photonic crystal configurations of PS, the double Bragg mirror structure is quite easy to prepare and exhibits interesting optical propert...

DNA strands that, mathematically speaking, are finite strings over the alphabet {A, G, C, T} are used in DNA computing to encode information. Due to the fact that A is Watson-Crick complementary to T and G to C, DNA single strands that are Watson-Crick complementary can bind to each other or to themselves in either intended or unintended ways. One of the structures that is usually undesirable f...

Deoxyribonucleic Acid (DNA) has certain unique properties such as selfassembly and self-complementary in hybridization, which are important in many DNAbased technologies. DNA computing, for example, uses these properties to realize a computation in vitro, which consists of several chemical reactions. Other DNA-based technologies such as DNA-based nanotechnology and polymerase chain reaction als...

We report a novel procedure, which can be applied to probing of specific DNA, for covalently attaching probe DNA to complementary sequences in double-stranded target DNA. Employing hairpin-like oligonucleotide probes in combination with successive use of recA protein and DNA ligase, probes can be attached directly to target DNA molecules without dissociation of the DNA. The hairpin-like structu...