Aspergillus niger is a highly potent fungus used in the production of alkaline protease. Extra cellular alkaline protease was purified from A. niger in a twostep procedure involving ammonium sulphate precipitation and Sephadex G100 column chromatography. The molecular mass of the enzyme was determined to be 60 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The ...

today bleach and detergents are being frequently used and some people use their mixture for more cleaning. because of chemical interaction of bleach and detergent, chlorine gas was released and thereby it could be dangerous for human health. this study examined the effects of exposed toxic mixture of bleach and detergent on the mucosa layer and lamina mucosa conjunctiva in the mice. in this stu...

The reversal of the inhibitory action of antiseptics by substances showing a specific affinity for the inhibiting agent has been reported for many compounds, the most striking effect probably being the reversal of the action of mercurials by H2S, glutathione, sodium thioglycolate, etc. Valko and DuBois (1944) have reported that the germicidal action of cationic detergents against both gram-posi...

It is well known that type II restriction enzyme activities and specificities can be modulated by altering solution conditions. The addition of co-solvents such as dimethyl sulfoxide (DMSO), alcohols and polyols can promote star activity, which is the cleavage of non-cognate sequences. While neutral detergents are often used to control protein aggregation, little is known about the effect of ne...

growth medium. Growth was not appreciably affected by the detergents (0.0001 %) except for dodecyl and octadecyl sulfate, which showed some inhibition. It is apparent that enzymatic activity decreases as the number of carbons in the alkyl chain is increased. Equivalent concentrations are without effect on preformed penicillinase. However, the same pattern of inhibition occurred when the deterge...

BACKGROUND Methylisothiazolinone (MI) contact allergy is severely affecting consumers with allergic contact dermatitis, owing to its presence in cosmetics, household detergents, and water-based paints, in particular. Data on the true isothiazolinone concentrations in these products are scarce, and labelling may be incorrect. OBJECTIVES To report on the MI concentrations in such products marke...