The Colorado potato beetle, Leptinotarsa decemlineata Say, is the major insect pest of potato, Solanum tuberosum L., in eastern North America and is renowned for resistance development, currently resistant to >40 insecticides worldwide. Host plant resistance may assist in delaying in resistance development to insecticides. We evaluated natural host plant resistance mechanisms (glandular trichom...

The paradox of successful invading species is that they are likely to be genetically depauperate compared to their source population. This study on Colorado potato beetles is one of the few studies of the genetic consequences of continent-scale invasion in an insect pest. Understanding gene flow, population structure and the potential for rapid evolution in native and invasive populations offer...

Bacillus thuringiensis EG2838 and EG4961 are highly toxic to Colorado potato beetle larvae, and only strain EG4961 is toxic to southern corn rootworm larvae. To investigate the cause of the different insecticidal activities of EG2838 and EG4961, cryIII-type genes toxic to coleopterans were cloned from each strain. The cryIIIB gene, cloned as part of an 8.0-kb EcoRI fragment of EG2838 DNA, encod...

The field experiment was carried out during 2004–2006. The aim of the study was to determine the influence of insecticides (Actara 25 WG – 0.08 kg/ha, Regent 200 SC – 0.1 L/ha, Calypso 480 SC – three rates: 0.05; 0.075; 0.1 L/ha), used for controlling Colorado potato beetle, on total glycoalkaloid (TGA) content in potato leaves and tubers of three cultivars (Mors, Wiking, Żagiel). The insectici...

The processing of olfactory information in the Colorado potato beetle, Leptinotarsa decemlineata Say, was studied by recording responses of olfactory neurones intracellularly in the deutocerebrum. Response characteristics of neurones in this first relay station of the olfactory pathway were measured when the antennae were stimulated with five general green leaf volatiles, i.e. cis-3-hexen-1-ol,...

Colorado potato beetles have been reared successfully through 12 generations on artificial diets containing either 2.5% potato leaf powder or 2.5% lettuce leaf powder/0.75% potato leaf powder. For all but one of the treatment groups, the mean duration of each of the four larval stages was between 0.8 and 1.5 days longer than the durations exhibited by control beetles that had been fed on potato...