G-protein coupled estrogen receptor (GPER) is a transmembrane that mediates non-genomic effects of estrogen. This study aimed to investigate the role GPER in stemness formation and malignancies triple negative breast cancer (TNBC) cells. Spheroids MDA-MB-468 cells were induced by mammosphere culture, proportion CD44+ /CD24−/low stem cell subpopulation was detected. Malignant characteristics, ex...

The appropriate dosage of edaravone—a radioprotective agent—and its effect on tumors are unknown. This study evaluated the effects edaravone intestinal injuries and in mice induced by whole body X-ray irradiation. Small mucositis was C3H/HeNSlc using a single dose (15 Gy). Edaravone (15, 30, 100 mg/kg) administered 30 min before irradiation to evaluate protective effect. After 3.5 days, jejunum...

PURPOSE Protein phosphatase 4 regulatory subunit 1 (PP4R1), as an interaction partner of the catalytic serine/threonine-protein phosphatase 4 catalytic subunit has been shown to involve in cellular processes and nuclear factor κB signaling. However, the functions of PP4R1 in human breast cancers remain unclear. This study is designed to explore the effect of PP4R1 knockdown on the biological ch...

UV inactivation, photoreactivation, and dark repair of Escherichia coli and Cryptosporidium parvum were investigated with the endonuclease sensitive site (ESS) assay, which can determine UV-induced pyrimidine dimers in the genomic DNA of microorganisms. In a 99.9% inactivation of E. coli, high correlation was observed between the dose of UV irradiation and the number of pyrimidine dimers induce...

Radioresistance is an important cause of cancer treatment failure. Circular RNAs (circRNAs) play crucial roles in development, including the radioresistance. This research aimed to determine function and related mechanism circ_0086720 radioresistance non-small cell lung (NSCLC). The expression circ_0086720, miR-375, Spindlin 1 (SPIN1) was measured using a quantitative real-time polymerase chain...

This study presents detection of free polyethylene glycol (PEG) and conjugated PEG in western blot assay using anti-PEG rabbit monoclonals specific to methoxy group of PEG. In a chromogenic western blot assay, detection limit (DL) for free and PEGylated protein was 2.5 μg and 0.5 μg, respectively. Using chemiluminescence substrate, DL of PEGylated granulocyte colony stimulating factor (GCSF) wa...

An in vitro B cell colony assay system was used to evaluate B cell growth from peripheral blood precursors in common acute lymphoblastic leukemia (CALL) patients in remission during maintenance therapy and in normal controls. Major differences between the two groups were found in the phenotypic and morphologic features of pooled colony cells. In both cases, the cells were E-. Controls' cells we...

Oxygen is essential for the survival of animals. Red blood cells in the circulation, i.e. peripheral erythrocytes, are responsible for transporting oxygen to tissues. The regulation of erythropoiesis in vertebrates other than mammals is yet to be elucidated. Recently we identified erythropoietin, a primary regulator of erythropoiesis, in Xenopus laevis, which should enable us to identify target...

In vitro radiation survival of peripheral blood T lymphocytes was studied in 15 clinically normal adults and 4 patients with Fanconi's anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (LST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) or...

Abstract Background Endometrial cancer is an invasive gynecological prevalent in the world. The pathogenesis of endometrial related to multiple levels regulation, referring oestrogen, tumor-suppressor gene (e.g. PTEN ) or microRNAs miR-23a and miR-29b). Metapristone a hormone-related drug, which widely used clinical treatment cancer. However, underlying regulatory mechanism metapristone on stil...