This study was designed to investigate the collagen metabolism and tyrosinase activity of Olibanum extracts (OE). The traditional effects are known to move blood, calm pain, solve oedema, and promote tissue regeneration. The effect of OE on type I procollagen production and collagenase (matrix metalloproteinase-1, henceforth referred as MMP-1) activity in human normal fibroblasts Hs68 after ult...

The regulation of M, 72,000 type IV collagenase and interstitial collagenase expression was studied in vitro. Three tumorigenic human cell lines were used, together with human fetal lung fibroblasts as a nontumorigenic control. M, 72,000 type IV collagenase was expressed constitutively by all four cell lines, whereas only A20S8 melanoma cells exhibited constitutive expression of interstitial co...

Clinical formulations of the antiarthritic drug aurothiomalate sometimes contain phenylmercury(ll) compounds as antimicrobial preservative agents and, in the presence of para-chloromercuri-benzoate, aurothiomalate is a potent inhibitor of collagenase. By (1)H NMR spectroscopy, para-hydroxymercuribenzoate and para-hydroxymercuriphenylsulphonate were shown to react with aurothiomalate by complexi...

We have shown previously that the expression of collagenase is upregulated in rabbit synovial fibroblasts cultured on a substrate of antibody to the alpha 5 chain of the alpha 5 beta 1 integrin fibronectin receptor or on the 120-kD cell-binding chymotryptic fragment of plasma fibronectin, but remains at basal levels in cells plated on intact plasma fibronectin. We now have identified some of th...

This study was designed to investigate the collagen metabolism and tyrosinase activity of Myrrh Extracts (ME). The currently known effects of ME are to move blood, calm pain, solve oedema, and promote tissue regeneration. The effect of ME on type I procollagen production and collagenase (matrix metalloproteinase-1, henceforth referred as MMP-1) activity in human normal fibroblasts Hs68 after ul...

As cancer cells traverse collagen-rich extracellular matrix (ECM) barriers and intravasate, they adopt a fibroblast-like phenotype and engage undefined proteolytic cascades that mediate invasive activity. Herein, we find that fibroblasts and cancer cells express an indistinguishable pericellular collagenolytic activity that allows them to traverse the ECM. Using fibroblasts isolated from gene-t...

An investigation into the methanol extracts obtained from stems of Dodonaea viscosa led to isolation one nor-clerodane diterpene (1) and two labdane diterpenes (2, 3), as well 17 known compounds (4–20). The structures these were elucidated based on chemical spectral evidence. stereochemical structure was confirmed via its circular dichroism spectrum calculated electronic spectrum. Isolated eval...

The corpus luteum (CL) is a transient endocrine organ that secretes progesterone to support pregnancy. The CL is formed from an ovulated follicle in a process that involves extensive angiogenesis and tissue remodeling. If fertilization does not occur or implantation is unsuccessful, the CL will undergo regression, which involves extensive tissue degradation. Extracellular proteases, such as ser...

Gene activation in eukaryotes requires chromatin remodeling, in part via histone modifications. To study the events at the promoter of a mitogen-inducible gene, we examined the induction of expression of the collagenase gene. It has been established that the collagenase gene can be activated by c-Jun and c-Fos and that the transcriptional coactivator p300 is involved in the activation. As expec...