This preliminary work explores a technique for processing collagen gels to provide a structured matrix support for cell growth and other tissue engineering applications without using cyto-toxic photo-initiators. Collagen gels can be structured by techniques similar to those of rapid manufacturing and retain the fibril structure of native collagen. Incorporation of alpha-modified minimal essenti...

Collagen plays a vital role in maintaining structural integrity and in determining tissue function. Therefore, methods to detect, quantify, and analyze collagen are valuable. Nevertheless, stains historically employed to detect collagen have disadvantages, principally a poor specificity for thin fibers. Conversely, picrosirius red, which has the capability to detect thin fibers, although freque...

Osteoarthritis (OA) is a multifactorial disease, associated with articular cartilage degeneration and eventually joint destruction. The phases of the disease have been described in detail, and mechanical factors play an important role in the initiation of OA, but many questions remain about its etiology. Swelling of cartilage, one of the earliest signs of damage, is proportional to the amount o...

Interest in constructing a reliable 3-dimensional (3D) collagen culture platform in microfabricated systems is increasing as researchers strive to investigate reciprocal interaction between extracellular matrix (ECM) and cells under various conditions. However, in comparison to conventional 2-dimensional (2D) cell culture research, relatively little work has been reported about the polymerizati...

Collagen V is a regulatory fibril-forming collagen that forms heterotypic fibrils with collagen I. Deletion of collagen V in the mouse is associated with a lack of fibril assembly in the embryonic mesenchyme, with a resultant lethal phenotype. The current work elucidates the regulatory roles of collagen V during development and growth of tissues. A conditional mouse model with a mutation in Col...

Abstract Collagen-based biomaterials with oriented fibrils have shown great application potential in medicine. However, it is still challenging to control the type I collagen fibrillogenesis ultrathin films. Here, we report an approach produce cohesive and well-organized films of about 10 nm thickness using Langmuir-Blodgett technique. Ellipsometry, rheology, Brewster angle microscopy are appli...

Introduction: Quantitative high-frequency ultrasound has been successfully used for the evaluation of articular cartilage e.g. reveal osteoarthritis related changes. During the degenerative process mechano acoustical properties of cartilage change: surface roughness, collagen and proteoglycan contents, and the density are affected. These changes can be detected as changes in variation of ultras...

Extracellular matrix-mimicking hydrogels with antioxidant, hemostasis, and antibacterial properties offer translating avenues towards wound repair through the promotion of fibroblast proliferation. In article number 2001122 by Hélder A. Santos, Mohammad-Ali Shahbazi, co-workers, hydrogen bonding among functional groups gelatin tannic acid creates a biocompatible hydrogel, where improvement re-e...

collagens are the most abundant proteins in the human body. their main function is to provide structural and mechanical support for the tissues, but they are also involved in a number of other biological functions including cell attachment, migration and differentiation. collagens and gelatins are widely used in pharmaceutical and medical applications. every year, more than 50,000 tons of colla...

INTRODUCTION The precise role of the remodeling process and possible therapies for bronchiolitis obliterans remain to be established. OBJECTIVE [corrected] In the present study, we sought to validate the importance of nasal collagen V tolerance to verify whether bronchovascular axis remodeling could be reverted by this therapeutic approach when compared to steroid treatment. METHODS Mice we...