نتایج جستجو برای: coated spermatozoa

تعداد نتایج: 61749  

Journal: :Reproduction 2013
D Rath S Barcikowski S de Graaf W Garrels R Grossfeld S Klein W Knabe C Knorr W Kues H Meyer J Michl G Moench-Tegeder C Rehbock U Taylor S Washausen

Pre-selection of spermatozoa based on the relative DNA difference between X- and Y-chromosome bearing populations by flow cytometry is an established method that has been introduced into commercial cattle production. Although several important improvements have increased the sort efficiency, the fertilising ability of sexed spermatozoa based on offspring per insemination is still behind farmers...

Journal: :Human reproduction 2011
A Perdrix A Travers M H Chelli D Escalier J L Do Rego J P Milazzo N Mousset-Siméon B Macé N Rives

BACKGROUND Spermatozoa with large vacuoles (SLV) may have a negative impact on embryo development. The origin of these vacuoles is unknown. We evaluated acrosome and nucleus alterations in isolated SLV, versus unselected spermatozoa. METHODS We studied 20 patients with teratozoospermia. Spermatozoa from the native semen sample and spermatozoa presenting a vacuole occupying >13.0% total head a...

Journal: :Molecular human reproduction 1997
S L Catt D Sakkas D Bizzaro P G Bianchi W M Maxwell G Evans

Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is still concern over its genetic safety due to the involvement of two potentially m...

2014
Akira Komiya Yoko Kawauchi Tomonori Kato Akihiko Watanabe Ichiro Tanii Hideki Fuse

We investigated sperm nuclear vacuolation in relation to acrosome reactions and the maintenance of sperm motility. Thirty male patients who visited our Male Infertility Clinic were enrolled. These patients underwent conventional semen analyses, Acrobeads tests, and high-magnification observation of the sperm head to evaluate the degree of nuclear vacuolation on the Acrobeads test scoring after ...

Journal: :Reproduction, fertility, and development 2017
Ai Ushiyama Atsushi Tajima Naoto Ishikawa Atsushi Asano

During cryopreservation, spermatozoa are subjected to cryodamage that leads to a decline in fertilisation ability. Due to the complex nature of this process, the initial trigger for cryodamage remains unknown. Recently, we demonstrated that cryopreservation induces early apoptotic changes characterised by phosphatidylserine (PS) translocation via sterol loss from the plasma membrane of chicken ...

2008
C. E. HAMNER WILLIAM L. WILLIAMS C. E. Hamner

A fourfold increase in oxygen uptake by rabbit spermatozoa incubated in the rabbit uterus and a twofold increase in cock sperm¬ atozoa incubated in the hen's oviduct over ejaculated spermatozoa is the first reproducible biochemical change to be demonstrated in sperma¬ tozoa residing in the female genital tract. It was shown that these rapidly respiring spermatozoa were capacitated because they ...

Journal: :Human reproduction 1997
B Baccetti E Strehler S Capitani G Collodel M De Santo E Moretti P Piomboni R Wiedeman K Sterzik

The effects of follicle stimulating hormone (FSH) treatment on the quality of human spermatozoa were assessed by examining the ultrastructure and the function of infertile human spermatozoa using a previously-defined formula. Using the spermatozoa as an andrological monitor shows that the therapeutic effect of FSH depends on the type of sperm defect. The response to FSH is, in many cases, posit...

Journal: :Human reproduction 2007
B Muciaccia S Corallini E Vicini F Padula L Gandini G Liuzzi A Lenzi M Stefanini

BACKGROUND Semen is the major vehicle for HIV-1 infection as it contains free and cell-associated virions and infected cells. However, the presence of HIV-1 in spermatozoa has been a matter of debate, since the sperm cell fraction may contain somatic infected cells that jeopardize the attribution of the detected virus to the spermatozoa. METHODS Spermatozoa from 12 HIV-1 seropositive subjects...

2017
Zhendong Zhu Zhanjun Ren Xiaoteng Fan Yang Pan Shan Lv Chuanying Pan Anmin Lei Wenxian Zeng

The process of cryopreservation results in over-production of reactive oxygen species, which is extremely detrimental to spermatozoa. The aim of this study was to investigate whether addition of cysteine to freezing extender would facilitate the cryosurvival of rabbit spermatozoa, and if so, how cysteine protects spermatozoa from cryodamages. Freshly ejaculated semen was diluted with Tris-citra...

Journal: :Human reproduction 1999
P Andolz M A Bielsa J Vila

A retrospective study was conducted in a large population to determine whether sperm quality has changed in Northeastern Spain between 1960 and 1996. From a total initial population of 22,759 men, two separate groups were studied: men with spermatozoa (n = 20,411) and those with azoospermia (n = 1364). After adjustment for age and sexual abstinence, multiple linear regression analyses were used...

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