The aim of the study was to characterise the free coagulase-negative Staphylococcus sp. strains isolated from normal (9.4%) and inflamed quarter milk samples of dairy cows in one herd localised in southern Poland. The isolates were examined phenotypically by cultural features, tube coagulase test, clumping factor (CF), and commercial agglutination test. Total number of 427 strains were cultivat...

Delta-hemolysin of coagulase-negative staphylococcal strains was immunologically identical to delta-hemolysin of Staphylococcus aureus (Newman) but distinct from delta-hemolysin from a canine strain of S. aureus.

Staphylococcus aureus and coagulase-negative staphylococcal isolates from various geographic areas in the United States were examined by using a conjugative transfer gene DNA probe in dot-blot hybridization assays. Of 175 S. aureus isolates, 47 (27%) hybridized with the probe, while 24 of 208 (11.5%) coagulase-negative staphylococci hybridized. However, among methicillin-resistant S. aureus 52%...

A new coagulase-negative species of the genus Staphylococcus, Staphylococcus pulvereri, was isolated from human and animal specimens. The complete 16S rRNA sequence of the type strain of S. pulvereri, NT215, was determined and compared with the sequences of 16S rRNAs from the other staphylococci. Strains of S. pulvereri were differentiated from other novobiocin-resistant Staphylococcus species ...

Background & Aims of the Study: Antibiotic resistance is an important problem in health care. The aim of this study was the survey of prevalence of bacteria isolated in a university affiliated hospital (Kerman, Iran) and detection of antibiotic resistance among major pathogens. Materials & Methods: In this cross-sectional study, during a 3-year period (from June 2006 to June 2009) 14699 sa...

gyrA mutations in quinolone-resistant pathogenic isolates of Staphylococcus spp. have been detected by the direct HinfI digestion of polymerase chain reaction products. Homology among gyrA genes allowed rapid examination of both coagulase-positive and -negative isolates. DNA sequence analysis revealed that ciprofloxacin resistance in Staphylococcus epidermidis is associated with a novel Ser-84-...

Aim and Objective: To determine the bacterial spectrum in diabetic foot lesions and analyze the antibiotic susceptibility pattern of the isolated bacteria. Methods and Methodology: Tissue samples/discharge/pus/ were cultured from 202 patients admitted for the treatment of diabetic foot infections. Specimens were tested by gram stain, culture and antibiotic sensitivity. Results: A total of 202 s...

Bovine mastitis is one of the major ill-health of cattle and buffaloes, responsible for great economic loss to dairy industry affecting livestock farmers worldwide. The major economic consequences of clinical bovine mastitis are due to treatment, loss in milk production, culling, changes in product quality and the risk of other diseases (Halasa et al., 2007). For etiological diagnosis of bovine...