Molecular typing of total 84 Staphylococcus aureus clinical isolates was performed using coagulase gene PCR. Out of 84 S. aureus strains total 33 different types of S. aureus strains were prevalent in this hospital and community. Types 2-7 and 9 were the most prevalent S. aureus strains accounting for more than 53% of total isolates. This technique is relatively inexpensive and is simple to per...

Misidentification of coagulase-negative staphylococci (CoNS) may delay appropriate treatment. We investigated 20 clinical isolates identified as Kocuria spp. by the Vitek 2 system. All were identified as CoNS by 16S rRNA gene sequencing (18 Staphylococcus epidermidis, 1 Staphylococcus haemolyticus). Four Kocuria isolates were shown to be identical to CoNS from the same patient by pulsed-field g...

conclusions methicillin-resistant staphylococcus aureus is an increasingly common cause of nosocomial infections. our results are in agreement with those of other studies reporting that a few specialized clones are responsible for most cases of mrsa nasal carriage. in this study, mrsa strains isolated from different wards of hospital were closely related when analyzed by coagulase gene typing. ...

Jay, James M. (Wayne State University, Detroit, Mich.). Production of lysozyme by staphylococci and its correlation with three other extracellular substances. J. Bacteriol. 91:1804-1810. 1966.-Lysozyme production was determined on plates containing 1 mg/ml of Lysozyme Substrate in Heart Infusion Agar with incubation at 37 C for 48 hr. Its production was compared with that of alpha-hemolysin and...

Staphylocoagulase, a protein produced by Staphylococcus aureus reactc with a plasma protein designated the Coagulase Reacting Factor (CRF) which is either prothrombin or is presumed to represent some active component of the prothrombin molecule."' The interaction of these two substances leads to the conversion of fibrinogen to fibrin, a reaction that proceeds in it*ro in the absence of calcium ...

A total of 398 consecutive clinical staphylococcus isolates, of which 205 were coagulase negative and 193 were coagulase positive, were tested in parallel by using AutoMicrobic system Gram-Positive Susceptibility-MIC cards and modified Mueller-Hinton agar containing 4% NaCl and oxacillin (6 micrograms/ml). The AutoMicrobic system cards correctly detected 103 of 104 (99%) oxacillin-resistant coa...