Background This study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (LH) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. MaterialsAndMethods Monolayers for co-culture were prepared from ovine oviduct epithelial cells (OOEC) in DMEM/F12 medium a...

Nowadays, most countries especially in arid and dry climate zones had experienced the water scarcity problems. In the agricultural sector, freshwater play a major role to supply water for the plant grows. Thus, the reuse of wastewater as alternative water resources in agricultural is highly recommended. However, wastewater is always contaminated despite after secondary treatment. Along benefici...

First report on the production of protease through fed batch cultivation using co-culture of Pseudomonas putida and Staphylococcus aureus from a crude carbon source, banana peel. Batch production by using synthetic medium, impact of some of the process parameters on cell growth and alkaline protease activity was investigated and the optimized values were found to be inoculum size of 5 % (v/v), ...