ined hemolysis (H)-indices before and after implementing the AC mode on a large number of samples. H-indices were determined photometrically at 405 and 700 nm (5 ). Retrospective examination of H-indices from 42 124 samples processed in the CC mode was compared with H-indices from 30 483 samples processed in the AC mode. The distributions of the different grades of hemolysis were identical for ...

Neutrophil extracellular traps are networks of DNA and associated proteins produced by nucleosome release from activated neutrophils in response to infection stimuli and have recently been identified as key mediators between innate immunity, inflammation, and hemostasis. The interaction of DNA and histones with a number of hemostatic factors has been shown to promote clotting and is associated ...

PURPOSE This study compared two methods of preparing platelet-rich plasma (PRP) gel and the levels of PDGF and TGFbeta in each preparation. MATERIALS AND METHODS Platelet-rich plasma gel was prepared by centrifugation and clotted using the ITA gelling agent (Natrex Technologies Inc, Greenville, NC) or by the addition of thrombin and calcium chloride. The levels of platelet-derived growth fact...

By JAMES G. WmTE, WILLIAM KRIVIT AND ROBERT VERNIER T HE PRIMARY PURPOSE of the complex mechanism of coagulation is to convert soluble fibrinogen molecules into a stable meshwork of fibrin. Appreciation of the macro-molecular architecture of fibrin is essential to an understanding of how clot formation fulfills its role in hemostasis. An electron microscopic study, combining the use of immune m...

The mechanism by which activated protein C stimulates fibrinolysis was studied in a simple radiolabeled clot lysis assay system containing purified tissue-type plasminogen activator, bovine endothelial plasminogen activator inhibitor (PAI), plasminogen, 125I-fibrinogen and thrombin. Fibrinolysis was greatly enhanced by the addition of purified bovine activated protein C; however, in the absence...

RATIONALE Glaucoma drainage implant surgery is a treatment option for the management of neovascular glaucoma. However, tube obstruction by blood clot after Ahmed glaucoma valve (AGV) implantation is an unpredictable clinically challenging situation. PATIENT CONCERNS-DIAGNOSES-INTERVENTIONS We report 4 cases using intracameral air injection for the prevention of the tube obstruction of AGV by ...

In response to injury, blood coagulation is activated and results in generation of the clotting protease, thrombin. Thrombin cleaves fibrinogen to fibrin which forms an insoluble clot that stops hemorrhage. Factor V (FV) in its activated form, FVa, is a critical cofactor for the protease FXa and accelerator of thrombin generation during fibrin clot formation as part of prothrombinase (1, 2). Ma...

recurrent pregnancy loss is usually defined as the loss of two or more consecutive pregnancies before 20 weeks of gestation, which occurs in approximately 5% of reproductive-aged women. it has been suggested that women with thrombophilia have an increased risk of pregnancy loss and other adverse pregnancy outcomes. thrombophilia is an important predisposition to blood clot formation and is cons...

Mitral balloon valvuloplasty which has been used for the treatment of rheumatic mitral stenosis (MS) for several decades can cause serious complications. Herein, we presented right atrial clot formation early after percutaneous mitral balloon valvuloplasty which was treated successfully with unfractioned heparin infusion.