A multiplex Taqman-based real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to identify potential severe strains of Citrus tristeza virus (CTV) and separate genotypes that react with the monoclonal antibody MCA13. Three strain-specific probes were developed using intergene sequences between the major and minor coat protein genes (CPi) in a multiplex reactio...

Citrus tristeza virus (CTV) is an important citrus pathogen causing considerable economic loss to production. Knowledge on genetic evolutionary of the CTV population in China remains limited. In this study, 1439 samples were collected from nine citrus-producing areas China. The coat protein (CP) genes amplified by RT-PCR, and sequenced analyze evolution. Analysis base composition showed AU pref...