Cervical cytological examination is the most widely applied screening method for cervical cancer and its precursors. Liquid-based cytology produces goodquality smears1 and enables the use of ancillary laboratory techniques to distinguish neoplastic from benign cells.1 Immunohistochemical and molecular markers have been tested to identify dysplastic cells in cervical smears. When testing for hum...

A practical assay, which exploits the maintenance of the cytoplasmic redox balance in live cells, has been developed to report in real time on relative cellular internalization rates of molecules derivatized as chromogenic disulfides.

A novel compound, N,N'-bis(2,4-di-sulfobenzyl)tolidine tetrasodium salt (SBT), was synthesized for use as a chromogenic indicator for oxidizing substances, and its applicability to the colorimetric determination of chlorine in water was examined.

PURPOSE The androgen receptor (AR) plays a central role in prostate cancer. Evidence from several groups indicates that epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) may enhance AR activity in prostate cancer cell lines. This study was designed to investigate the protein expression of AR, EGFR, and HER2 and to determine whether the EGFR and HER2 gen...

A genomic library of Lactobacillus delbrueckii ssp. lactis DSM7290 in the low copy number vector pLG339, was screened for the presence of peptidase genes. Using the chromogenic substrate gly-ala-beta-naphthylamide, which is not a substrate for any of the recently cloned peptidases of DSM7290, and the multiple peptidase deficient Escherichia coli strain CM89, allowed the isolation of clones, whi...

A chromogenic and fluorogenic detection of a nerve agent simulant was developed based on diethyl chlorophosphate triggered tandem phosphorylation and intramolecular cyclization of N-(rhodamine B)-deoxylactam-2-aminoethanol.

Expansion microscopy (ExM) is a methodology which increases the imaging resolution of sample through expansion prior to optical by crosslinking it hydrogel, digesting it, and swelling gel, thereby expanding sample. Though many variations ExM have been created, all these rely on labeling with fluorescent markers. Here we use enzyme-linked antibody markers generate chromogenic staining proteins i...

Infections caused by extended-spectrum beta-lactamase (ESBL)- and ampC beta-lactamase-producing gram-negative bacteria complicate therapy and limit treatment options. Several different panels for ESBL detection with automated systems exist. In addition, a chromogenic agar medium is available for ESBL screening. We compared two automated identification and susceptibility testing systems with reg...

Plasma antithrombin levels were measured by clotting, immunological, and amidolytic methods on two groups of subjects: 20 normal individuals and nine patients studied serially post-operatively (hip replacement). The postoperative patients were observed for the emergence of deep-vein thrombosis using 125I-fibrinogen uptake measurements (FUT). The three methods gave similar ranges for the normal ...

BACKGROUND The analysis of co-localized protein expression in a tissue section is often conducted with immunofluorescence histochemical staining which is typically visualized in localized regions. On the other hand, chromogenic immunohistochemical staining, in general, is not suitable for the detection of protein co-localization. Here, we developed a new protocol, based on chromogenic immunohis...