A new simple, precise and accurate reverse phase HPLC method was developed and validated for the estimation of vinblastine in pharmaceutical formulations using the mobile phase 90% methanol, 5% acetonitrile and 5% of 0.1% orthophosphoric acid and the pH was maintained at 3.5. The resultant chromatogram obtained has a high resolution and low tailing factor (1.29). The linearity curve showed a co...

We investigated the use of a diode array detector in conjunction with isocratic cation-exchange liquid chromatography for detection of organic acids in urine. The spectra and retention times of abnormal peaks found on chromatography of urine from patients with methylmalonic aciduria, maple syrup urine disease, and lactic aciduria were recorded and compared with those obtained for group of pure ...

A sample of 0.104 M nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC-MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction...

This paper describes a novel approach for analysis of mercury speciation in fish using gas chromatography coupled with microwave-induced plasma optical emission spectrometry (GC-MIP-OES) in surfatron resonant cavity. Sample treatment was based on quantitative leaching of mercury species from fish tissue with ultrasound-assisted acidtoluene extraction. The extracted mercury species analyzed with...

Naphyrone sample diluted in methanol was analysed with a previously described method (9) with a Dionex (Camberley, U.K.) HPLC system (P580 binary pump, STH 585 column oven, GINA 50 autosampler and a PDA-100 diode array detector). Data acquisition was by a Dionex Chromeleon Version 6.8 software package with the DAD recording spectral data between 200 nm and 595 nm. Wavelengths of 210 nm and 220 ...

Ozonated olive oil was prepared by bubbling ozone-oxygen gas through olive oil until it solidified. Hand C-NMR spectra of the ozonated oil showed that all C-C double bonds in triacyglycerols were converted to ozonide. The spectra also showed no other structural change and the absence of degraded products of ozonide such as aldehydes and carboxylic acids. HPLC chromatogram of the oil showed thre...

Submit Manuscript | http://medcraveonline.com Abbreviations: SCs: Synthetic Cannabinoids; Δ-9-THC: Delta9-TetraHydraCannabinol; UHPLC: Ultra High Performance Liquid Chromatography; DAD: Photodiode Array; MS: Mass Spectrometry. GC: Gas Chromatography; DART: Direct Analysis in Real Time; TOF: Time of Flight Spectrometry; NMR: Nuclear Magnetic Resonance; ATR: Attenuated Total Resonance; FTIR: Four...

A method for the titration of antigens based on separation by filtration through millipore filters of the complex of antigen with fluorescein isothiocyanate-labeled antibodies from free labeled anti­ bodies and nonspecific y-globulins is described. The fluorescence of the antigen-antibody complex is measured directly on the millipore filters using the Turner fluorometer equipped with a paper ch...

A change in paper shape is described for use in descending paper chromatography. The "flask-shaped" paper provides greater reliability in the identification of amino acids in unknown mixtures. As many as three samples of unknown and/or known components can be run simultaneously on the same paper and under the same conditions. The method has been used by other investigators for the separation of...