Escherichia coli O157:H7 outbreaks associated with produce consumption have brought attention to livestock manures and manure-based soil amendments as potential sources of pathogens for the contamination of these crops. Procedures for enumeration of E. coli O157:H7 are needed to assess the risks of transmission from these manures and their by-products. A direct plating method employing spiral p...

Background: The yeasts of Candida genus represent the 4th cause sepsis. However, they are rarely found in infections central nervous system. diagnosis is difficult and often delayed, worsening prognosis. Methods: A 47-year-old patient was admitted at emergency military hospital Tunis, Tunisia for SARS-COV-2 infection. After 4 days non-amelioration, a Lumbar Puncture (LP) performed culture on Sa...

 Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene to detection of MRSA. A total of 220 clinical isolates of S. aureus which w...

Abstract Background Candida species is considered one of the normal inhabitant commensal microbiota human body. However, it can also act as an opportunistic pathogen especially in hospitals (nosocomial infection) and among immunocompromised patients. The accurate, precise, rapid reliable identification to level great importance for control management candidiasis. Results One hundred eighteen di...

Polysaccharide monooxygenases (PMOs) and Glycoside Hydrolases (GH) play a crucial role in breaking down chitin cellulose. Research on PMOs has mainly focused their the production of bioethanol from biomass. Still, potential as virulence factors microorganisms is not been fully studied. Some pathogenic bacteria secrete PMOs, which have identified factors. The ability Vibrio species to metabolize...