The feasibility of capillary electrophoresis frontal analysis for the study of low-molecular-weight ligandchondroitin sulfate interactions was investigated. The interaction of the 7 cationic ligands (lidocaine, propranolol, scopolamine, N-methyl scopolamine, N-butyl scopolamine, 2-propylisochinolinium, and methyl viologen) with the glycosaminoglycan chondroitin sulfate was investigated in 0.067...

Glycosaminoglycan was isolated from the body wall of sea cucumber Stichopus japonicus by a method consisting of enzymatic digestion, gel filtration, and ion-exchange chromatography. One gram of sea cucumber glycosaminoglycan was composed of 2.50 mmol of sulfate, 0.47 mmol of N-acetylgalactosamine (GalNAc), 0.53 mmol of glucuronic acid (GlcA), 1.73 mmol of fucose, and a small amount of peptide. ...

Oversulfated chondroitin sulfate (OSCS) was identified as a contaminant in certain heparin preparations as the cause of adverse reactions in patients. OSCS was found to possess both plasma anticoagulant activity and the ability to activate prekallikrein to kallikrein. Differentially sulfated chondroitin sulfates were prepared by synthetic modification of chondroitin sulfate and were compared to...

Chondroitin sulfate (CS) is a linear acidic polysaccharide, composed of repeating disaccharide units of glucuronic acid and N-acetyl-D-galactosamine and modified with sulfate residues at different positions, which plays various roles in development and disease. Here, we chemo-enzymatically synthesized various CS species with defined lengths and defined sulfate compositions, from chondroitin hex...

Scaffold, as one of the components for bone tissue engineering, requires formulated biomaterials that are both structurally and compositively similar to composition. Among others, chitosan, gelatin chondroitin sulfate known potential candidates scaffold composites can be easily obtained from waste-based resources. This study aims investigate cytotoxicity different composition concentration regi...

Recently, we described a peptide-modified AAV2 vector (AAV-GMN) containing a capsid-displayed peptide that directs in vivo brain vascular targeting and transduction when delivered intravenously. In this study, we sought to identify the receptor that mediates transduction by AAV-GMN. We found that AAV-GMN, but not AAV2, readily transduces the murine brain endothelial cell line bEnd.3, a result t...

Treatment of day-4 chick embryos with 6-aminonicotinamide (6-AN) impairs limbs chondrogenesis and produces micromelia. Interference with limb cartilage development may be related to decreased NAD-dependent synthesis of ATP due to the fact that chondrogenesis is dependent upon anaerobic metabolism. To better understand the effect of 6-AN on chondrogenesis, isolated cartilage epiphyses from day-1...

Chondroitin sulfate occurs in bovine nasal cartilage as compounds with protein called proteinpolysaccharides (PP) of which there are two kinds: (a) the major part is not sedimented at 100,000 X g and is called PP-L; (b) the smaller part is readily sedimented at 10,000 X g and is called PP-H (1). Both, on treatment with NaOH (0.15 in, 25”, 16 hours), yield chondroitin sulfate in amounts correspo...