ACARA, MARGARET, AND BARBARA RENNICK. Rqylation of plasma choline by the renal tub&: bidirectional transport of choline. Am. J. Physiol. 225(5) : 1123-I 128. 1973-Choline is a biocation with a pK, of 13.5 which is actively transported by the organic cation transport system in the renal tubule. A radiochemical assay using choline kinase was adapted to measure free choline in plasma and urine wit...

Legionella dumoffii is an intracellular pathogen of freshwater protozoans capable infecting and multiplying in mammalian cells, causing a severe respiratory disease called Legionnaires’ disease. The pathomechanism infection development very complex depends on many factors, including the structure properties macromolecules that build components L. cell envelope. Phospholipids (PLs) forming biolo...

The sensitive, specific growth response to choline activity of the thermophilic enteric yeast Torulopsis pintolopessi enables estimation of free and bound choline activity in rat and human fluids and tissues- as little as 10 ng/ml of choline is measurable. Unlike other microbial assays, estimation of unbound (free) choline activity is not influenced by methionine or phospholipids. The method al...

OBJECTIVE Topical cytidine-5'-diphosphocholine (CDP-choline) improves functional recovery and promotes nerve regeneration in sciatic nerve injury in rats. The aims of this study were to test whether systemic treatment with CDP-choline was effective in improving the recovery of injured sciatic nerve, and to determine whether the cytidine and/or choline moieties of CDP-choline contribute to its ...

Objective(s): This study aimed to compare the diagnostic values of 11C-choline and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT) in patients with cholangiocarcinoma (CCA). Methods: This prospective study was conducted on 10 patients (6 males and 4 females), aged 42-69 years, suspected of having CCA based on CT or magnetic resonance imaging (MRI) resu...

Inhibitors of protein synthesis capable of differential effects on nascent peptide synthesis on membrane-bound and free polyribosomes were employed to investigate the structure and function of cellular membranes of liver. The formation of membranous whorls in the cytoplasm and distension of nuclear membranes were induced by inhibitors of protein synthesis (i.e., cycloheximide and emetine) which...

Generation of H2O2 by rat liver mitochondria with choline, glycerol 1-phosphate and proline as substrates has been shown by using high-concentration phosphate buffer. Rates obtained under these conditions were higher and more consistent as compared with the earlier reports with high-concentration mannitol/sucrose/Tris buffer. Sulphate ions could replace phosphate indicating a requirement for a ...