BACKGROUND The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. METHODS The 398-bp sequence of a gene that code...

Three serious cholera epidemics have threatened the world during the last 10 years. As a countermeasure against such cholera epidemics, three vaccines, CVD 103-HgR, WC/rBS, and Vietnamese WC, showed good performance. CVD 103-HgR is a recombinant attenuated live vaccine for travelers, and its highly safety and protective efficacy have been demonstrated in volunteers in advanced countries. WC/rBS...

Background: The newly emerged coronavirus, SARS-CoV-2, the causative agent of COVID-19 disease, appeared in Wuhan, China December 2019 and led to death millions people. pandemic has increased demand for effective vaccines treatments worldwide. Due changes virus genome caused by mutation, variants with higher transmission ability pathogenicity have raised excessive concerns about efficiency deve...