نتایج جستجو برای: cholera toxin b subunit ctb

تعداد نتایج: 1022452  

Journal: :Immuno 2022

Bacterial lipopolysaccharides (LPS), also referred to as endotoxins, are major outer surface membrane components present on almost all Gram-negative bacteria and determinants of sepsis-related clinical complications including septic shock. LPS acts a strong stimulator innate or natural immunity in wide variety eukaryotic species ranging from insects humans specific effects the adaptive immune s...

Journal: :Experimental neurology 2003
Marcelino Avilés-Trigueros Sergio Mayor-Torroglosa Antonio García-Avilés María P Lafuente María E Rodríguez Jaime Miralles de Imperial María P Villegas-Pérez Manuel Vidal-Sanz

In adult rats, we have induced retinal ischemia and investigated anterogradely labeled surviving retinal ganglion cell (RGC) afferents to the contralateral superior colliculus (SC). The animals received topically in their left eyes two 5-microl drops of saline or saline-containing 0.5% brimonidine (BMD), 1 h before 90 min of retinal ischemia induced by ligature of the left ophthalmic vessels. T...

2013
Priya Saikumar Lakshmi Dheeraj Verma Xiangdong Yang Bethany Lloyd Henry Daniell

Tuberculosis (TB) caused by Mycobacterium tuberculosis is one of the leading fatal infectious diseases. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, three candidate antigens, ESAT-6 (6 kDa early secretory antigenic target) and Mtb72F (a fusion polyprotein from two TB antigens, Mtb32 and Mtb39) fused with ch...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2010
Heidi L Lujan Gurunanthan Palani Jean D Peduzzi Stephen E DiCarlo

Individuals with spinal cord injuries above thoracic level 6 (T(6)) experience episodic bouts of life-threatening hypertension as part of a condition termed autonomic dysreflexia. The paroxysmal hypertension can be caused by a painful stimulus below the level of the injury. Targeted ablation of mesenteric projecting sympathetic neurons may reduce the severity of autonomic dysreflexia by reducin...

2014
Ho-Hsien Lee Irene Cherni HongQi Yu Raimund Fromme Jeffrey D. Doran Ingo Grotjohann Michele Mittman Shibom Basu Arpan Deb Katerina Dörner Andrew Aquila Anton Barty Sébastien Boutet Henry N. Chapman R. Bruce Doak Mark S. Hunter Daniel James Richard A. Kirian Christopher Kupitz Robert M. Lawrence Haiguang Liu Karol Nass Ilme Schlichting Kevin E. Schmidt M. Marvin Seibert Robert L. Shoeman John C. H. Spence Francesco Stellato Uwe Weierstall Garth J. Williams Chunhong Yoon Dingjie Wang Nadia A. Zatsepin Brenda G. Hogue Nobuyuki Matoba Petra Fromme Tsafrir S. Mor

CTB-MPR is a fusion protein between the B subunit of cholera toxin (CTB) and the membrane-proximal region of gp41 (MPR), the transmembrane envelope protein of Human immunodeficiency virus 1 (HIV-1), and has previously been shown to induce the production of anti-HIV-1 antibodies with antiviral functions. To further improve the design of this candidate vaccine, X-ray crystallography experiments w...

Journal: :Journal of neuroscience methods 1996
A Angelucci F Clascá M Sur

We report an improved immunohistochemical protocol for revealing anterograde axonal transport of the subunit B of cholera toxin (CTB) which stains axons and terminals in great detail, so that single axons can be followed over long distances and their arbors reconstructed in their entirety. Our modifications enhance the quality of staining mainly by increasing the penetration of the primary anti...

2012
Andreas Boberg Alexandra Stålnacke Andreas Bråve Jorma Hinkula Britta Wahren Nils Carlin

We increase our understanding of augmenting a cellular immune response, by using an HIV-1 protease-derived epitope (PR75-84), and variants thereof, coupled to the C-terminal, of the B subunit of cholera toxin (CTB). Fusion proteins were used for immunizations of HLA-A0201 transgenic C57BL/6 mice. We observed different capacities to elicit a cellular immune response by peptides with additions of...

2013
Jingjing Cui Lijuan Ha Xinlong Zhu Fuchun Wang Xianghong Jing Wanzhu Bai

The purpose of this study is to investigate the specific innervations on "Weizhong" (BL40) and "Huantiao" (GB30) by using a dual neural tracing technique. After Alexa Fluor 488 and 594 conjugates of cholera toxin subunit B (AF488/594-CTB) were, respectively, injected into BL40 and GB30 in the same rat, the labeled sensory and motor neurons were examined in the rat's dorsal root ganglia (DRGs) a...

2011
Cin L Thang Bushansingh Baurhoo Joyce I Boye Benjamin K Simpson Xin Zhao

BACKGROUND Cow's milk allergy (CMA) is one of the most prevalent human food-borne allergies, particularly in infants and young children from developed countries. Our study aims to evaluate the effects of Lactobacillus rhamnosus GG (LGG) administration on CMA development using whole cow's milk proteins (CMP) sensitized Balb/C mice by two different sensitization methods. METHODS LGG supplemente...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 1999
F R Morales S Sampogna J Yamuy M H Chase

The present study was undertaken to identify trigeminal premotor interneurons that become activated during carbachol-induced active sleep (c-AS). Their identification is a critical step in determining the neural circuits responsible for the atonia of active sleep. Accordingly, the retrograde tracer cholera toxin subunit B (CTb) was injected into the trigeminal motor nuclei complex to label trig...

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