Analysis of chloroplast DNA varia­ tion in species of Lycopersicon (Solan­ aceae) has demonstrated the utility of the techniques for assessing phyloge­ netic relationships among species (Pal­ mer and Zamir, 1982). A similar but more extensive survey has been initi­ ated in the genus Atriplex (Chenopo­ diaceae) using the same techniques. Atriplex is a genus of over 400 de­ scribed species and ha...

background:confirmation of olive oil authenticity and particularly virgin olive oil has a great importance. several advanced chemical and genetic analyses have been used to monitor especial components; however each has its limitations especially when detecting hazelnut-adulterated olive oil. objectives:the objective of this research is to assess the presence of trace amount of hazelnut oil in o...

Electron-microscope autoradiography has been used to obtain information on the localization of DNA labelled with [3H]thymidine in chloroplasts known to be replicating and concomitantly synthesizing and segregating DNA, in cultured leaf disks. The studies were made using both Microdol-X developer and a 'compact' developer which gave a smaller grain size. About 80% of the grains were associated w...

Organisms must have efficient mechanisms of DNA repair and recombination to prevent alterations in their genetic information due to DNA damage. There is evidence for DNA repair and recombination in plastids of higher plants, although very little is known at the biochemical level. Many chloroplast proteins are of eubacterial ancestry, suggesting that the same could be true for the components of ...

Toc12 is a novel J domain-containing protein identified in pea (Pisum sativum) chloroplasts. It was shown to be an integral outer membrane protein localizing in the intermembrane space of the chloroplast envelope. Furthermore, Toc12 was shown to associate with an intermembrane space Hsp70, suggesting that Toc12 is important for protein translocation across the chloroplast envelope. Toc12 shares...

Benzyladenine (BA) treatment was found to induce chloroplast DNA (ctDNA) synthesis after it had stopped in primary leaves of light-grown intact bean plants (Phaseolus vulgaris L.). The leaves were treated with BA from 7 days after sowing. Chloroplasts were isolated and the ctDNA content per chloroplast was determined. Chloroplast division occurred until 13 days after sowing in untreated leaves....

DNA gyrase, which catalyzes topological transformation of DNA, plays an essential role in replication and transcription in prokaryotes. Virus-induced gene silencing of NbGyrA or NbGyrB, which putatively encode DNA gyrase subunits A and B, respectively, resulted in leaf yellowing phenotypes in Nicotiana benthamiana. NbGyrA and NbGyrB complemented the gyrA and gyrB temperature-sensitive mutations...

The systematic utility of sequences from a non-coding region of chloroplast DNA (cpDNA) between psbA and trnH (6v6) was examined by assessing phylogenetic relationships in subtribe Sonchinae (Asteraceae: Lactuceae). Primers constructed against highly conserved regions of tRNA genes were used for PCR amplification and sequencing. The psbA-trnH intergenic spacer contains several insertions and de...