An efficient, nanoliter-scale microfabricated bioprocessor integrating all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis, has been developed and evaluated. Hybrid glass-polydimethylsiloxane (PDMS) wafer-scale construction is used to combine 250-nl reactors, affinity-capture purification chambers, high-performance capillary electrophoresis cha...

the built up layer thickness in secondary deformation zone is one of the important parameters in metal cutting process. the built up layer (bul) is formed in second deformation zone near the tool-chip interface in the back of the chip. this parameter influences the tool life and machined surface quality. this bul should not be confused with the built up edge (bue). the deformation of the bul in...

Microsatellite analysis is an important tool in clinical research and molecular diagnostics because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10-15% of colorectal, gastric and endometrial carcinomas are associated with MSI, and this has an impact on clinical prognosis. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250, recommende...

Ultrafast laser writing of waveguides in glasses is a very flexible and simple method for direct on-chip integration of photonic devices. In this work we present a monolithic optofluidic device in fused silica providing label-free and spatially-resolved sensing in a microfluidic channel. A Mach-Zehnder interferometer is inscribed with the sensing arm orthogonally crossing the microfluidic chann...

We introduce an automated immunoblotting method that reports protein electrophoretic mobility and identity in a single streamlined microfluidic assay. Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three PA gel elements into a glass microfluidic chip achieved multiple functions, including (1) rapid protein separation via on...

nowadays network-on-chips is used instead of system-on-chips for better performance. this paper presents a new algorithm to find a shorter path, and shows that genetic algorithm is a potential technique for solving routing problem for mesh topology in on-chip-network.

A glass microchip is described in which reagents and serum samples for competitive immunoassay of serum theophylline can be mixed, reacted, separated, and analyzed. The device functions as an automated microfluidic immunoassay system, creating a lab-on-a-chip. Electroosmotic pumping was used to control first the mixing of 50-fold-diluted serum sample with labeled theophylline tracer in a 1:1 ra...

To realize the immense potential of large-scale genomic sequencing after the completion of the second human genome (Venter's), the costs for the complete sequencing of additional genomes must be dramatically reduced. Among the technologies being developed to reduce sequencing costs, microchip electrophoresis is the only new technology ready to produce the long reads most suitable for the de nov...

The built up layer thickness in secondary deformation zone is one of the important parameters in metal cutting process. The built up layer (BUL) is formed in second deformation zone near the tool-chip interface in the back of the chip. This parameter influences the tool life and machined surface quality. This BUL should not be confused with the built up edge (BUE). The deformation of the BUL in...

as technology scales deep into the nanometer regime, on-chip communication becomes more susceptible to transient noise sources, such as crosstalk, external radiation, and spurious voltage spikes. the network on chip s modularity and reusability has brought about the use of error control methods to address transient errors in network on chip links.in this work, we design a fault tolerance router...