1. Chickens inoculated subcutaneously with 0.2 cc. of a 10(-2) to 10(-7) dilution of Western equine mouse brain virus had the virus in the blood serum between the 12th and the 48th hour in most instances. The fowls showed no signs of illness. 2. Viremia could be induced regularly in chickens by inoculating subcutaneously the least amount of virus which would produce encephalitis in the mouse wh...

Taiwan red-feathered country chickens (TRFCCs) are one of the main meat resources in Taiwan. Due to the lack of any systematic breeding programs to improve egg productivity, the egg production rate of this breed has gradually decreased. The prediction by zone (PreZone) program was developed to select the chickens with low egg productivity so as to improve the egg productivity of TRFCCs before t...

The measurement of chicken and human antibodies to Clostridium botulinum neurotoxins A, B, and E was accomplished by affinity isolation of complexes containing these antibodies. By this approach, a mixture of toxin with the test antibody, fluoresceinated antibody, and enzyme (Russell's viper venom factor X activator)-labeled antibody is allowed to form a complex in solution phase. This complex ...

We have identified, by a combination of ligand, 45Ca2+, and immunoblotting, two large membrane proteins akin to the mammalian so-called low density lipoprotein (LDL) receptor-related protein (LRP) in chicken tissues. LRP has thus far been demonstrated only in mammalian species where it is thought to act as a receptor for proteinase-alpha 2-macroglobulin complexes and/or chylomicron remnants, li...

Serum from Rous sarcoma virus tumor-bearing rabbits immunoprecipitated from extracts of the freshwater sponge Spongilla lacustris a tyrosine-specific protein kinase with characteristics similar to the chicken pp60c-src kinase activity. An immune competition assay confirmed the relationship between the protein from sponges and viral pp60v-src.

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

In 1937 Heidelberger and Pedersen (1) mixed assayed amounts of antigen (G) and antibody (A), and used ultracentrifugal analysis to determine the amount of free component, antigen or antibody, in the system. Somewhat later these experiments were extended by Pappenheimer, Lundgren, and Williams (2) with diphtheria toxin and its equine antibody to the point at which it could be demonstrated that t...