Antibodies to three isolates each of Armillaria mellea, A. ostoyae, A. tabescens, and Lentinula edodes were isolated from eggs of immunized laying hens. The reactivity of each antibody preparation with all isolates was examined using an enzyme-linked immunosorbent assay (ELISA). The cross-reactivity of the antibody preparations to a given Armillaria species varied considerably when tested again...

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

BACKGROUND Vaccines constitute a unique selective pressure, different from natural selection, drives the evolution of influenza virus. In this study, A/Chicken/Shanghai/F/1998 (H9N2) was continually passaged in specific pathogen-free embryonated chicken eggs with or without selective pressures from antibodies induced by homologous maternal antibodies. Genetic mutations, antigenic drift, replica...

West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.

The occurrence of different types of intermediate filaments in primary cultures of cells and in cultured cell lines was studied by the indirect immunofluorescence (IFL) technique. The antibodies used were spontaneous monoclonal human antibodies of immunoglobulin M (IgM) class against vimentin-type intermediate filaments (fibroblast 58 x 10 mol. wt subunit protein), and experimental rabbit antib...

Monospecific antibodies to chicken gizzard actin, alpha-actinin, and filamin have been used to localize these proteins at the ultrastructural level: secondary cultures of 14-d-old chicken embryo lung epithelial cells and chicken heart fibroblasts were briefly lysed with either a 0.5% Triton X-100/0.25% glutaraldehyde mixture, or 0.1% Triton X-100, fixed with 0.5% glutaraldehyde, and further per...