When transferred to suspension culture on agarose-coated dishes, dedifferentiated chick embryo chondrocytes resume the chondrocyte phenotype and continue their maturation to hypertrophic chondrocytes (Castagnola, P., G. Moro, F. Descalzi Cancedda, and R. Cancedda. 1986. J. Cell Biol. 102:2310-2317). In this paper we report the identification, purification, and characterization of a low molecula...

Continuous surveillance of influenza was carried out in Pune between 1978 and 1990. Most of the cases were identified during investigation of 16 outbreaks of influenza in Pune over this period. The majority of cases were children. Ten of the outbreaks occurred during rainy seasons. A total of 290 isolates consisting of several antigenic variants of influenza type A (H3N2), type A (H1N1), and ty...

Two bilaterally symmetric eyes arise from the anterior neural plate in vertebrate embryos. An interesting question is whether both eyes share a common developmental origin or they originate separately. We report here that the expression pattern of a new gene ET reveals that there is a single retina field which resolves into two separate primordia, a suggestion supported by the expression patter...

OBJECTIVE To determine the survival of chick embryos after ethanol vapour exposure by noting the number of dead and alive embryos and comparing with age-matched controls. STUDY DESIGN Experimental study. PLACE AND DURATION OF STUDY Department of Anatomy at the regional centre of College of Physicians and Surgeons, Islamabad, from February 2006 to February 2007. METHODOLOGY Chicken eggs, p...

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and initial patterning of the nervous system. This video demonstrates how to graft organizer tissue into a host, a method by which Hensen s node (the organizer in the chick embryo) is grafted to a host compete...

A glycoprotein resembling ovomucoid was purified from chorioallantoic membranes of 12.5 day chick embryos by a sequential procedure involving zinc chloride precipitation, hydroxylapatite chromatography, and ion exchange chromatography on DEAEand CM-cellulose. The resulting preparation was homogeneous as indicated by polyacrylamide gel electrophoresis at 3 pH values, isoelectric focusing, ultrac...

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A major cell surface protein, CSP, of chick embryo fibroblasts has been shown to constitute up to 3% of total cell protein, and to be decreased after viral transformation. Its role in normal cell behavior is not known. We have isolated CSP from chick embryo fibroblasts by extraction with 1 M urea and find that these preparations of CSP agglutinate formalinized sheep erythrocytes at protein conc...