Lysine acetyltransferases (KATs) play a critical role in the regulation of transcription and other genomic functions. However, a persistent challenge is the development of assays capable of defining KAT activity directly in living cells. Toward this goal, here we report the application of a previously reported dCas9-p300 fusion as a transcriptional reporter of KAT activity. First, we benchmark ...

In this paper a globally stabilizing boundary feedback control law for an arbitrarily fine discretization of a nonlinear PDE model of a chemical tubular reactor is presented. The objective is to stabilize an unstable steady–state of the system using boundary control of temperature and concentration on the inlet side of the reactor. We discretize the original nonlinear PDE model in space using f...

Chemical process control textbooks and courses differ significantly from their electrical or mechanical-oriented brethren. It is our experience that colleagues in electrical engineering (EE) and mechanical engineering (ME) assume that we teach the same theory in our courses and merely have different application examples. The primary goals of this article are to i) emphasize the distinctly chall...

T under intensive management are often subject to outbreaks of infectious diseases. Diseases usually are most damaging when weather or cultural conditions favor the disease-causing agent but not plant growth and vigor. Cultural conditions that enhance turfgrass diseases include close mowing, inadequate or excessive nitrogen fertility, light and frequent irrigations, excessive thatch, poor drain...

Field horsetail is a perennial, rhizomatous weed with summer-growing fern-like foliage and sporelating stems. It likes moist, free-draining sandy soils and gravel riverbeds and flood plains. Glasshouse experiments on 10-month old potted field horsetail plants showed excellent efficacy of imazapyr, amitrole, metsulfuron, picloram and combinations of picloram with metsulfuron or triclopyr, all at...

Control structure design deals with the structural decisions of the control system, including what to control and how to pair the variables to form control loops. Although these are very important issues, these decisions are in most cases made in an ad hoc fashion, based on experience and engineering insight, without considering the details of each problem. In the paper, a systematic procedure ...

The paper shows one approach to control of a nonlinear process represented by an isothermal continuous stirredtank reactor. The hybrid adaptive controller designed with the use of a polynomial synthesis, LinearQuadratic approach and spectral factorization was used for controlling of the product‘s concentration via change of the volumetric flow rate of the reactant. An adaptivity of the system i...

In this paper, a globally stabilizing boundary feedback control law for an arbitrarily fine discretization of a nonlinear PDE model of a chemical tubular reactor is presented. A model that assumes no radial velocity and concentration gradients in the reactor, the temperature gradient described by use of a proper value of the effective radial conductivity, a homogeneous reaction, the properties ...

The adaptive output feedback control problem of chemical distributed parameter systems is investigated while the process parameters are unknown. Such systems can be usually modeled by semilinear partial differential equations (PDEs). A combination of Galerkin’s method and proper orthogonal decomposition is applied to generate a reduced order model which captures the dominant dynamic behavior of...

Biological and chemical process performance is largely determined by reactor performance. The stage for product quality and process efficiency and capacity is set by reaction yield and selectivity. The burden and difficulty for downstream separation and purification processes is minimized by better reactor control. In this tutorial the factors affecting reactor performance are described with ex...