نتایج جستجو برای: cfp 10 protein

تعداد نتایج: 2127958  

Journal: :Chembiochem : a European journal of chemical biology 2007
Zihao Cheng Mark Miskolzie Robert E Campbell

The conventional approach for developing folded peptides involves chemical synthesis of systematically modified peptide variants and individual characterization by circular dichroism and NMR spectroscopy. The use of synthetic peptides and reliance on low-throughput characterization techniques necessarily restricts the sequence diversity that can be explored, though efforts have been made to ove...

Journal: :PLoS ONE 2008
Laura Savolainen Liana Pusa Hwa-Jung Kim Heidi Sillanpää Ilkka Seppälä Tamara Tuuminen

BACKGROUND In recent years T cell based interferon gamma release assays (IGRA) have been developed for immunodiagnosis of M. tuberculosis infection. At present these assays do not discriminate between disease and latency. Therefore, more promising antigens and diagnostic tools are continuously being searched for tuberculosis immunodiagnostics. The heparin binding hemagglutinin (HBHA) is a surfa...

2010
Heidi J. Chial Peter Lenart Yong Q. Chen

BACKGROUND Human APPL1 and APPL2 are homologous RAB5 effectors whose binding partners include a diverse set of transmembrane receptors, signaling proteins, and phosphoinositides. APPL proteins associate dynamically with endosomal membranes and are proposed to function in endosome-mediated signaling pathways linking the cell surface to the cell nucleus. APPL proteins contain an N-terminal Bin/Am...

Journal: :Infection and immunity 1999
Y A Skeiky M J Lodes J A Guderian R Mohamath T Bement M R Alderson S G Reed

Culture filtrate proteins (CFP) of Mycobacterium tuberculosis have been shown to contain immunogenic components that elicit at least partial protective immunity against Mycobacterium infection. To clone genes encoding some of the immunogenic proteins, we made a high-titer rabbit anti-CFP serum and used it to screen an M. tuberculosis genomic expression library in Escherichia coli. In this paper...

Journal: :Journal of cell science 2005
Rihong Zhou Lixin Zhu Andrew Kodani Paul Hauser Xuebiao Yao John G Forte

Phosphorylation of the membrane-cytoskeleton linker protein ezrin has been functionally linked to acid secretion and vesicle recruitment to the apical secretory membrane in gastric parietal cells. Phosphorylation of the conserved T567 residue of ezrin has been shown to alter the N/C oligomerization of ezrin and promote the formation of actin-rich surface projections in other cells. To test the ...

Journal: :Circulation research 2007
Seth L Robia Kenneth S Campbell Eileen M Kelly Zhanjia Hou Deborah L Winters David D Thomas

Phospholamban (PLB) or the sarcoplasmic reticulum Ca2+-ATPase (SERCA) were fused to cyan fluorescent protein (CFP) and coexpressed with PLB fused to yellow fluorescent protein (YFP). The expressed fluorescently tagged proteins were imaged using epifluorescence and total internal reflection fluorescence microscopy. YFP fluorescence was selectively bleached by a focused laser beam. CFP fluorescen...

2016
Shaohui Fan Fengying Guan Xingliang Xu David I. Forrester Xiaolu Tang

Converting secondary natural forests (SFs) to Chinese fir plantations (CFPs) represents one of the most important (8.9 million ha) land use changes in subtropical China. This study estimated both biomass and soil C stocks in a SF and a CFP that was converted from a SF, to quantify the effects of land use change on ecosystem C stock. After the forest conversion, biomass C in the CFP (73 Mg ̈ha ́1)...

Journal: :Chemistry 2010
Ulyana Angel Shimanovich Devorah Matas Shulamit Michaeli Artur Cavaco-Paulo Aharon Gedanken

This paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co-workers who have developed a method by which high-intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water-insoluble liquids.1 By using high-intensity ultrasound, we have...

Journal: :Infection and immunity 1999
D C Dillon M R Alderson C H Day D M Lewinsohn R Coler T Bement A Campos-Neto Y A Skeiky I M Orme A Roberts S Steen W Dalemans R Badaro S G Reed

We have used expression screening of a genomic Mycobacterium tuberculosis library with tuberculosis (TB) patient sera to identify novel genes that may be used diagnostically or in the development of a TB vaccine. Using this strategy, we have cloned a novel gene, termed mtb39a, that encodes a 39-kDa protein. Molecular characterization revealed that mtb39a is a member of a family of three highly ...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2007
Claire Goldsbury Edda Thies Sven Konzack Eva-Maria Mandelkow

We describe a procedure for quantifying the numbers of expressed fluorescent fusion proteins on vesicles transported in axons. The method can be used to estimate numbers of vesicle-anchored molecules moving in both anterograde and retrograde directions and is also applicable to cytosolic proteins. This is demonstrated using neurons (explanted retinal ganglion cells) transfected with yellow fluo...

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