biodegradation of phenanthrene (phe) was studied in aqueous culture to demonstrate the potential of the mixed culture in degrading high concentration of phe. the experiments were conducted to monitor biodegradation of phenanthrene for duration of 6 days. biodegradation of phe was successfully achieved in low and middle concentration by the isolated mixed culture. a full factorial central compos...

in this study, we investigated the effects of osmotic process with or without ultrasound on solid gain (sg) and water loss (wl) of cranberries. response surface methodology was used to model and determine the optimum processing conditions for wl and sg during osmotic dehydration of samples. sucrose (40-60%) and salt (0-8%) concentrations, temperature (30-50°c) and frequency of ultrasound (0-130...

Plackett and Burman statistical design was applied to screen the nutritional factors affecting β-galactosidase production by Lactobacillus fermentum CM33. Accordingly, lactose, tryptone and Tween80 were found to be the positive effective factors at the significant level above 80%. Central composite design (CCD) and response surface plot predicted that the maximum enzyme activity of 49.81 U/100 ...

Studies on Co-Culture fermentation of tapioca flour as the substrate symbiotic strains of starch digesting Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces Cerevisaie in a batch fermentation. The effect of Carbon to Nitrogen (C/N) ratio of the fermentation medium on ethanol concentration and biomass was investigated. The optimum C/N ratio of the fermentation medium ...

The catalytic action of two non commercial lipases (E.C. 3.1.1.3) in the synthesis of isoamyl acetate in a solvent-free system, were tested using an experimental design in order to better understand the relationship between the reaction variables. Yields ranging from 67 to 77 % molar conversion were achieved. The synthesis of isoamyl acetate catalyzed by the chosen lipase (Rhizopus sp.) was opt...

Extracellular fungal cellulases are key enzymes for the degradation of lignocellulosic biomass. Greater production of these enzymes could reduce the cost of biofuels production. In this study, the basal medium for cellulase production by a Penicillium janthinellum mutant (EU2D-21) in submerged fermentation conditions was optimized using response surface methodology (RSM). Initial studies using ...

This study examined the effect of cell-wall-degrading enzymes added to temper water on wheat milling performance and flour quality. An enzyme cocktail consisting of cellulase, xylanase, and pectinase and five independent variables (enzyme concentration, incubation time, incubation temperature, tempered wheat moisture content, and tempering water pH) were manipulated in a response surface method...

A quadratic central composite design (CCD) in response surface methodology was applied to explicate the combinational concentration of effectors that influence fermentative cellulase production by Brevibacillus parabrevis. It was examine that 10% inoculums ((7X10/ml)) concentration and 37C temperature was optimum for cellulase production after 24 h of fermentation. Concentration of effectors (g...

Arginine metabolism in Enterococcus faecium sp. GR7 was enhanced via arginine deiminase pathway. Process parameters including fermentation media and environmental conditions were optimized using independent experiments and response surface methodology (central composite design). Fermentation media (EAPM) were optimized using independent experiments which resulted in 4-fold increase in arginine ...

esterification of chitosan with adipic acid catalyzed by immobilized candida antarctica lipase b was carried out in this study. response surface methodology (rsm) based on a four-factor- five-level small central composite design (sccd) was employed to model and analyze the reaction. a total of 21 experiments representing different combinations of the four reaction parameters including ch...