Marine microalga, Scenedesmus sp., which is known to be suitable for biodiesel production because of its high lipid content, was subjected to the conventional Folch method of lipid extraction combined with high-pressure homogenization pretreatment process at 1200 psi and 35°C. Algal lipid yield was about 24.9% through this process, whereas only 19.8% lipid can be obtained by following a convent...

Objective: The current study tries to elucidate the impact of low-affinity Pi transporter Pho91 (using pho91Δ cells) on lipid metabolism and cellular organelles morphology (mitochondria vacuole) in yeast Saccharomyces cerevisiae. Methods: profile was performed using thin layer chromatography (TLC), membrane defect determined DiOC6 staining, droplets (LDs) were observed by Nile red mitochondrial...

c-Abl is a non-receptor tyrosine kinase whose activity is tightly controlled in vivo through unknown mechanisms. Recent studies suggest that c-Abl may be regulated in different cellular contexts by distinct lipids.

Aquatic organisms are exposed to multiple stressors in the environment, including contaminants and rising temperatures due climate change. The objective of this study was characterize effect increased temperature on chemical-induced toxicity lipid profiles during embryonic development hatch fish. This is important because many environmental chemicals modulate cellular metabolism lipids, both wh...

The present study is motivated by the increasing demand to consider internal partitioning into tissues instead of exposure concentrations for environmental toxicity assessment. To this end, physiologically based pharmacokinetic (PBPK) models can be applied. We evaluated variation in accuracy PBPK model outcomes depending on tissue constituents modeled as sorptive phases and chemical distributio...

Mothers of the Atlantic grey seal, Halichoerus grypus , lactate for about 20 days, during which they do not feed and may have no access to water. Following weaning, depart sea leaving their pups unattended unfed up another 40 days. We are interested in how this lactation strategy supports pups’ rapid growth development while also preparing them long fast before independently going sea. report a...

The purpose of the present study was to examine the effects of exogenous cholesterol on the apolipoprotein (Apo) B gene expression in HepG2 cells. Pure cholesterol had no significant effect on either the cellular content of cholesteryl esters or the net accumulation of neutral lipids and ApoB in the culture medium. By contrast, addition of 25-hydroxycholesterol increased the net accumulation of...

To be effective for cytoplasmic delivery of therapeutics, nanoparticles (NPs) taken up via endocytic pathways must efficiently transport across the cell membrane and subsequently escape from the secondary endosomes. We hypothesized that the biomechanical and thermodynamic interactions of NPs with plasma and endosomal membrane lipids are involved in these processes. Using model plasma and endoso...

High density lipoprotein (HDL) phospholipids and apolipoproteins remove cellular lipids by two distinct mechanisms, but their relative contribution to reverse cholesterol transport is unknown. Whereas phospholipid-mediated cholesterol efflux from cultured cells reflects the activity of the HDL receptor SR-BI, apolipoprotein-mediated lipid removal is regulated in response to changes in cellular ...