The study of individual cells with infrared (IR) microspectroscopy often requires living cells to be cultured directly onto a suitable substrate. The surface effect of the specific substrates on the cell growth-viability and associated biochemistry-as well as on the IR analysis-spectral interference and optical artifacts-is all too often ignored. Using the IR beamline, MIRIAM (Diamond Light Sou...

Fifteen putative Streptomyces spp. isolated from soil were selected to be analysed using Fourier transform infrared (FT-IR) spectroscopy and 16S rRNA gene sequencing. Four colour groupings (groups 1–4) were obtained and described according to the colour of their substrate mycelia, aerial mycelia, spore mass and pigmentation. The dendrogram constructed using unsupervised cluster analysis of the ...

Fourier transform infrared (FT-IR) spectroscopy is a rapid, noninvasive technique with considerable potential for application in the food and related industries. We show here that this technique can be used directly on the surface of food to produce biochemically interpretable "fingerprints." Spoilage in meat is the result of decomposition and the formation of metabolites caused by the growth a...

Cancer is a disease that does great harms to the health of human beings. FT-IR spectroscopy could identify variability at the molecular level in biological specimens. It is a rapid and noninvasive method, which could be used intraoperatively to modify surgical procedures. The aim of this paper is to identify and separate cancer from colitis in endoscopic colon biopsies through the use of FT-IR ...

The analysis of volatile compounds for food products characterization is currently performed by gas chromatographic separation, often coupled with mass spectrometry. In our research the possibility of the application of the Fourier-transform infrared spectroscopy (FT-IR) technique to the cheese volatile fraction evaluation was investigated. An Evolved Gas Analysis (EGA) FT-IR prototype, develop...

This study aimed to evaluate metabolic fingerprinting by Fourier transform infrared (FT-IR) spectroscopy as a technique for investigating microbial communities and their activities in soil. FT-IR spectra from earthworm casts, and other ‘biosamples’, were compared using multivariate cluster analyses. The work formed part of a wider study to quantify the risk of horizontal gene flow and to assess...

Quercetin is a bioflavonoid, used to treat several diseases. The aim of the present work was to estimate the effect of quercetin loaded chitosan nanoparticles (QUER-CHIT) against monocrotophos induced toxicity in rat erythrocytes in vitro. Scanning electron microscopy (SEM), atomic force microscopy (AFM), x-ray diffraction (XRD) and fourier transform infrared (FT-IR) characterization and biocom...

Biodegradable poly(ethyleneglycol)-poly(valerolactone)-poly(ethyleneglycol) [PEG-PVL-PEG] copolymers were synthesized through ring opening polymerization of δ-valerolactone (VL) followed by the coupling of monomethoxy poly(ethyleneglycol-poly(valerolactone) (mPEG-PVL) with hexamethylene diisocyanate (HDI). The copolymers were characterized by (1)H NMR, FT-IR, and GPC. Block copolymers of PEG an...

This article presents a novel method for selective acquisition of Fourier transform infrared (FT-IR) spectra of microorganisms in-line during fermentation, using Saccharomyces cerevisiae as an example. The position of the cells relative to the sensitive region of the attenuated total reflection (ATR) FT-IR probe was controlled by combing a commercially available ATR in-line probe with contact-f...

Abstract Carbon quantum dots (CQDs) were prepared throught hydrothermal synthesis method. The TEM and FT-IR spectra showed the successful of CQDs well water solubility CQDs. After co-cultured with human breast cancer cells (MCF-7 cells), stained emitted stronge green fluorescence, indicated that had a good application prospect in cell imaging.