the gas separation performance of commercial ceramic titania and hybrid silica (hybsi®) membranes was assessed using the gas permeation and permporometry methods. results indicated that the hybsi® membranes have a hybrid surface containing regions covered by a polymeric matrix and others with inorganic pores. these membranes have high h2 selectivity, which increases significantly with temperatu...

We report on an electrochemically driven CO2 separation process employing commercial anion exchange membranes to directly remove from a dilute gas mixture and transport it across cell. This m...

Our current understanding of cell sorting relies on physical difference, either in the interfacial properties or motile force, between cell types. But is such asymmetry a prerequisite for cell sorting? We test this using a minimal model in which the two cell populations are identical with respect to their physical properties and differences in motility arise solely from how cells interact with ...

The purification of immune cell populations is often required in order to study their unique functions. In particular, molecular approaches such as real-time PCR and microarray analysis require the isolation of cell populations with high purity. Commonly used purification strategies include fluorescent activated cell sorting (FACS), magnetic bead separation and complement depletion. Of the thre...

BACKGROUND INFORMATION Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MACS (magnetic-activated cell sorting). The findings show that the cells are mul...

Forensic DNA analysis of sexual assault evidence requires separation of DNA from epithelial (victim) and sperm (perpetrator) cells. The conventional method used by crime laboratories, which is termed "differential extraction", is a time-consuming process. To supplant the conventional process, separation of sperm from a biological mixture containing epithelial cells has been demonstrated on a mi...

A one-step immunomagnetic separation technique was performed on a microfluidic platform for the isolation of specific cells from blood samples. The cell isolation and purification studies targeted T cells, as a model for low abundance cells (about 1:10,000 cells), with more dilute cells as the ultimate goal. T cells were successfully separated on-chip from human blood and from reconstituted blo...

BACKGROUND Circulating tumor cells (CTCs) in blood circulation may play a role in monitoring and even in early detection of metastasis patients. Due to the limited presence of CTCs in blood circulation, viable CTCs isolation technology must supply a very high recovery rate. METHODS Here, we implement design of experiments (DOE) methodology in order to optimize the Bio-Ferrography (BF) immunom...

OBJECTIVE To develop a reliable technique to enrich for rare cells in blood suspensions using only negative selection steps including a flow-through immunomagnetic cell separations system and by optimizing variables normally encountered during such enrichment processes. METHODS A human breast cancer cell line was cultivated and spiked at a ratio of 1 cancer cell to 10(5) total leukocytes in b...

  Unsteady flow separation on the airfoils with local flexible membrane (LFM) has been investigated in transient and laminar flows by the finite volume element method. A unique feature of the present method compared with the common computational fluid dynamic softwares, especially ANSYS CFX, is the modification using the physical influence scheme in convection fluxes at cell surfaces. In contr...