tissue culture is an attractive alternative for plant propagation. micropropagation is a technique to ensure a constant and uniform source of ornamental plants. matthiola incana is an important ornamental species mainly cultivate by seed. matthiola incana seeds were germinated on solid ms medium without plant growth regulators. shoot proliferation and root formation are possible using kinetin (...

An efficient protocol was developed for the induction of callus, somatic embryogenesis and regeneration response of two sugarcane varieties: HSF-243 and HSF-245, in this study. Callus was induced on young leaf explants cultured on Murashige and Skoog’s medium supplemented with different concentration of 2, 4-D singly or in combination with BAP. The highest percentage of callus induction was obs...

leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...

Aim: The primary aim of the present study was to screen salt tolerant calli and optimization in-vitro regeneration protocol from selected calli.
 Methodology: cotyledonary leaf explants sterilized by using 1% Bavistin, 0.1% Mercuric chloride 70% ethanol followed washing with distilled water. Sterilized were cultured on Murashige Skoog (MS) medium supplemented different concentration NaCl (...

Tissue culture is an attractive alternative for plant propagation. Micropropagation is a technique to ensure a constant and uniform source of ornamental plants. Matthiola incana is an important ornamental species mainly cultivate by seed. Matthiola incana seeds were germinated on solid MS medium without plant growth regulators. Shoot proliferation and root formation are possible using kinetin (...

background: the water-soluble extract of ganoderma lucidum (reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. objective: to investigate the effect of ganoderma lucidum (g. lucidum) on cytokine production by mice peritoneal macrophages. methods: mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold pbs. per...

Background: The water-soluble extract of Ganoderma lucidum (Reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. Objective: To investigate the effect of Ganoderma lucidum (G. lucidum) on cytokine production by mice peritoneal macrophages. Methods: Mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold PBS. Per...

The present study was conducted to investigate the effects of different concentrations and combinations of plant growth regulators (PGRs) on callus induction of Tecomella undulata (Sm) Seem. The leaf segments were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins such as NAA, 2,4-D, IAA and cytokikinins like BAP and KIN alone a...

BACKGROUND There are considerable attempts worldwide on herbal and traditional compounds to validate their use as anti-cancer drugs. Plants from Moringaceae family including Moringa oleifera possess several activities such as antitumor effect on tumor cell lines. In this study we sought to determine if callus and leaf extracts of M. oleifera possess any cytotoxicity. MATERIALS AND METHODS Eth...