The chemical modification of two new double-headed protease inhibitors from black-eyed peas, a trypsin-chymotrypsin inhibitor (BEPCI) and a trypsin inhibitor (BEPTI) with dansyl chloride was investigated under various conditions. The NH,-terminal serine of both BEPCI and BEPTI, the 4 lysyl residues of BEPCI, and 4 of the 5 lysyl residues of BEPTI, could not be dansylated in the absence of urea....

We describe here a novel approach to the dissection of chromatin structure by extracting DNA fragments from digested nuclei irreversibly immobilized (via proteins) on Celite columns. Three successive gradients (NaCl, LiCl-urea, temperature) are used to release three families of DNA fragments: namely, the 'DNA adherence' classes DNA-0, DNA-I and DNA-II, respectively. This 'protein image' DNA chr...

Extraction of house-fly heads with neutral phosphate buffer yielded a dark brown solution from which a number of pigments were separated, either wholly or partially, by chromatography on a column of calcium phosphate mixed with celite. One of the pigments was light-sensitive, and had a yellow color, with a spectral absorption maximum at 437 mmicro in phosphate buffer at pH 6.5. Several consecut...

A penicillin amidase, obtained from the exogenous medium of a Bacillus megaterium culture, was purified approximately 96-fold by means of two cycles of adsorption on, and elution from, Celite, followed by a further fractionation on carboxymethylcellulose. On the basis of sedimentation centrifugation analysis, the final preparation was deemed to be homogeneous with an apparent molecular weight o...

General experimental details. All organometallic and catalytic procedures were carried out under an inert atmosphere of argon by using a dual manifold vacuum/argon line and standard schlenk techniques, or in an MBraun glove box. All solvents were predried by refluxing for three days under dinitrogen over the appropriate drying agents (sodium for toluene; potassium for THF; sodium-potassium allo...

In an attempt to fractionate Cg acids produced by Ascaris with the aid of partition chromatography, it was noted that one fraction had distribution characteristics resembling those of n-valeric acid (2). Elution of this fraction began earlier than, but was completed simultaneously with, that of n-Valerie acid. Therefore, it appeared that this fraction may consist of at least two components. Evi...

Sol-gel entrapment is an efficient immobilization technique that allows preparation of robust and highly stable biocatalysts. Lipase from Candida antarctica B was immobilized by sol-gel entrapment and by sol-gel entrapment combined with adsorption on Celite 545, using a ternary silane precursor system. After optimization of the immobilization protocol, the best enzyme loading was 17.4 mg/g supp...

Microbial cells of Pseudomonas delafieldii were coated with magnetic Fe3O4 nanoparticles and then immobilized by external application of a magnetic field. Magnetic Fe3O4 nanoparticles were synthesized by a coprecipitation method followed by modification with ammonium oleate. The surface-modified Fe3O4 nanoparticles were monodispersed in an aqueous solution and did not precipitate in over 18 mon...

1. Glycopeptides and glycolipids were isolated from normal cat liver and liver from a cat affected with GM1 gangliosidosis. 2. Bio-Gel P-6 chromatography of the crude glycopeptide fractions demonstrated three major peaks of hexose-containing compounds that were greatly increased in the mutant liver sample; these peaks contained oligosaccharides that comprised over 2% of the liver wet weight. 3....

The general procedure for the isolation of the phosphate carrier from mitochondria involves solubilization with non-ionic detergents and chromatography on hydroxylapatite [l-3]. With high resolution SDS-gel electrophoresis this preparation can be separated into 4-5 protein bands [2]. Further purification, in particular removal of the ADP/ATP-carrier, was obtained by chromatography on Celite [2]...