Tobacco mosaic virus infection of Nicotiana tabacum mesophyll protoplasts did not affect the pattern of chloroplast or total cellular DNA synthesis for at least 120 h when compared with that of mock-infected cells. Calli derived from infected protoplasts often showed large amounts of tobacco mosaic virus RNA and coat protein.

Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymovi...

Accepted : August, 2009 The complexity of many virus diseases has led to the development of a large number of approaches for their control. The use of chemical insecticides at large scale has caused serious environmental problems. However, permanent elimination of any significant amount of broad spectrum synthetic pesticide also cannot be adopted. Thus new pesticides which can meet different st...

RNA of Tobacco Mosaic Virus is shown to be able to migrate into isolated mouse liver mitochondria, whence it can be reisolated intact. The migration of RNA is accompanied by enhanced rate of protein synthesis, which is sensitive to chloramphenicol, but not to cycloheximide. Evidence is presented showing that, among the products formed is the coat protein of Tobacco Mosaic Virus.

Celery (Apium graveolens) is a regularly consumed vegetable, providing strong, distinct flavours to dishes as well health benefits. Constituents of the aroma profile celery include range volatile compounds (terpenes, phthalides and aldehydes) that contribute its characteristic odour flavour. Vast amount research has been completed on celery. However, there limited information stating cultivar, ...

Background: Spine gourd (Momordica dioica Roxb. Willd) is one of the important cucurbitaceous crops grown across the world for vegetable and medicinal purposes. Diseases caused by the DNA viruses are becoming the limiting factors for the production of spine gourd reducing its potential yield. For the commercial cultivation of the spine gourd, propagation material used by most o...

In vitro RNA synthesis by tobacco mosaic virus and cowpea chlorotic mottle virus replicase were inhibited by cordycepin triphosphate. Inhibition could be overcome with higher concentrations of ATP in assay mixtures but not with UTP. Products synthesized in vitro by tobacco mosaic virus RNA replicase in the presence of inhibitor revealed replicative form but not replicative intermediate RNAs. Th...

alfalfa mosaic virus (almv) is one of the most conspicuous alfalfa viruses in the world. in order to identify the distribution, host range and molecular characterization of alfalfa mosaic virus in kerman province, 514 alfalfa samples showing mosaic, calico, mottle and malformation symptoms as well as 66 potato samples showing calico symptoms were collected from alfalfa and potato fields in kerm...