A complete cDNA clone encoding the human acrosin-trypsin inhibitor HUSI-II has been isolated from a cDNA library of human testis and completely sequenced. The cDNA of 594 bp contained an open reading frame of 252 base pairs. The deduced amino acid sequence comprised the complete amino acid sequence of HUSI-II and a putative signal peptide. Northern blotting analysis revealed that HUSI-II is syn...

A 1173-base pair cDNA encoding bovine cellular retinaldehyde-binding protein (CRALBP) was cloned from a bovine retinal cDNA expression library using as probes both anti-CRALBP polyclonal and monoclonal antibodies. The amino acid sequence deduced from the cDNA corresponds exactly to that determined by direct analysis of NH2-terminally acetylated bovine CRALBP (Crabb, J. W., Johnson, C. M., Carr,...

After the publication of our work in BMC Cancer [1], we became aware of an omission. In the Methods section, under the subtitles "Cell lines and tissue samples for cDNA arrays" and "cDNA probe preparation" we failed to quote the original description of how the cDNA library was generated, published in the Journal Archives of Medical Research as quoted below [2]. Since this initial analysis, furt...

We have isolated several cDNA clones encoding delta-aminolevulinate dehydrase [ALAD; porphobilinogen synthase; 5-aminolevulinate hydro-lyase (adding 5-aminolevulinate and cyclizing), EC 4.2.1.24], the second enzyme in the heme biosynthetic pathway. We used a rabbit polyclonal antibody developed against the purified 35-kDa subunit of rat liver ALAD to screen a lambda gt11 cDNA expression library...

We screened zebra finch cDNA libraries with the plaque-based competitive hybridization technique for genes differentially expressed in the brains of courting and non-courting males. The cDNA libraries were generated by the SMART TM cDNA Library Construction Kit. Sixty-six percent (35/53) of the isolated clones possessed high homology to sequences present in the cDNA libraries of species ranging...

Overexpression of the 92 kDa type IV collagenase (MMP-9) contributes to cancer progression. However, to date, there are few known regulators of expression of this metalloproteinase. We employed an expression library comprising 500,000 cDNA clones to screen for novel regulators of MMP-9 expression. HT1080 cells were transiently co-transfected with an MMP-9 promoter-luciferase reporter and pools ...

A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. L...

We present the complete nucleotide sequence of an Aplysia californica actin cDNA gene isolated from an abdominal ganglion cDNA library (Miles.M. and Scheller.R.H., Stanford University; unpublished) (Fig. 1). Using PCR technology, we showed that this actin cDNA is expressed in the sheath surrounding the neurons (probably in the muscular cells of the sheath), while another homologous actin gene i...

BACKGROUND AND PURPOSE Transitional cell carcinoma has been diagnosed mostly in urinary bladder in southern Taiwan and has an exceptionally high mortality rate. To identify the genes associated with bladder cancer, we investigated differential gene expression. Six bladder tumor cDNA libraries were constructed and their sequences were compared and analyzed. METHODS mRNA from bladder tumor cell...

Name of the Sequence. Red seabream β-actin cDNA. Genus and Species. Pagrus major. Origin of Clone. Total RNA was isolated from ovary of red seabream. Poly(A)+ RNA was purified using an Oligotex-dT30 (Super) mRNA purification kit (Takara, Japan). Double-stranded complementary DNA synthesis was carried out using a TimeSaver cDNA synthesis kit (Pharmacia Biotech, Uppsala, Sweden). The cDNA library...