Despite intense recent interest, the suppressive mechanisms of regulatory CD25+CD4 T cells remain poorly understood. One deficiency in the field is the lack of in vivo models where the effects of regulatory CD25+CD4 T cells on antigen-specific responder T cells can be measured quantitatively. We describe one such model here. We compared responses of adoptively transferred naive wild-type antige...

Costimulation blockade with the B7-CD28 pathway-specific agent belatacept is now used in clinical kidney transplantation, but its efficacy remains imperfect. Numerous alternate costimulatory pathways have been proposed as targets to synergize with belatacept, one of which being the inducible costimulator (ICOS)-ICOS ligand (ICOS-L) pathway. Combined ICOS-ICOS-L and CD28-B7 blockade has been sho...

CD8 T cells stimulated with a suboptimal dose of anti-CD3 Abs (100 pg/ml) in the presence of IL-15 retain a naive phenotype with expression of CD45RA, CD28, CD27, and CCR7 but acquire new functions and differentiate into immunosuppressive T cells. CD8+CCR7+ regulatory T cells (Tregs) express FOXP3 and prevent CD4 T cells from responding to TCR stimulation and entering the cell cycle. Naive CD4 ...

In response to viral infection, unprimed naive CD8(+), major histocompatibility complex class I-restricted, virus-specific T cells clonally expand and differentiate into memory- and effector-type cells. Changes in CD8(+) subset distribution were studied in 17 subjects with acute human immunodeficiency virus type 1 infection and in 14 subjects with acute Epstein-Barr virus (EBV) infection, with ...

We performed an international meta-GWAS of psoriasis, increasing effective sample size by 3.4-fold. Using COJO, we identified 191 independent psoriasis loci at genome-wide (GW) significance (179 outside the MHC). conducted eQTL analysis 1,195 strand-specific RNA-seq libraries passing QC (MQ30, ∼25M reads/sample) from 9 FACS-purified immune cell types (CD4/CD8, CLA-/CLA+, and 0h/24 h CD3-CD28-ac...

Signals delivered by costimulatory molecules are implicated in driving T cell expansion. The requirements for these signals, however, vary from dispensable to essential in different infections. We examined the underlying mechanisms of this differential T cell costimulation dependence and found that the viral context determined the dependence on CD28/B7-mediated costimulation for expansion of na...

Naturally arising CD4(+)CD25(+)FoxP3(+) regulatory T cells (nTregs) have an essential role in maintenance of immune homeostasis and peripheral tolerance. Previously, we reported that conventional CD4(+) and CD8(+) T cells undergo p53-induced CD28-dependent apoptosis (PICA) when stimulated with a combination of immobilized anti-CD3 and anti-CD28 Abs, whereas nTregs expand robustly under the same...

IntroductionHepatocellular carcinoma (HCC) is the most common primary malignant tumor of liver.AimThis study aimed to assess serum hTERT level and its relation progression liver disease. Also, it effect HCV core protein on memory T-cells in patients with or without HCC, correlation between cells phenotype disease same patients.MethodsHTERT was assessed through relative quantitative RT-PCR. Flow...

Antitumor immune response is usually inhibited by immunosuppressive tumor microenvironment. CD8+CD28T lymphocyte (Ts; or CD8+ Treg) is another regulatory T cell apart from CD4+CD25highCD127low Treg, and it has been detected infiltrating in tumor tissue of various types as well. The aim of this study is to investigate possible role of both Tregs in peripheral circulation of non small cell lung c...