The present study was conducted on efficiency of utilization of dietary energy for milk production in lactating crossbred cattle. 18 lactating crossbred cattle of early to mid-lactation, approximate body weight (375.39±23.43 kg), milk yield, parity and stage of lactation were divided into three groups of six animals each and were fed 0, 50 and 100% diammonium phosphate (DAP) in the mineral mixt...

OBJECTIVES To examine the clinical value of six seminal plasma components in the evaluation of sperm quality and in the differential diagnosis of men with infertility. METHODS We analyzed 202 seminal plasmas for prostate-specific antigen, glucose, pepsinogen C, insulin-like growth factor binding protein-3, prostaglandin D synthase (PGDS), and BRCA1-like immunoreactive protein (BRCA1-LIP) usin...

A meta-analysis was undertaken to examine the effects of different supplementation strategies on production beef cattle grazing tropical pastures during wet and dry seasons in Brazil. The database built with 132 studies published from 1999 2010, which accounted for 6,275 individual animals. supplements assessed were classified into three groups: energy supplement containing <20% crude protein (...

Introduction: Leptin is a critical metabolic signal linking nutrition and reproductive functions. Leptin receptors are expressed in germ cells, Sertoli and Leydig cells. Oxidative stress increases sperm DNA damages. Nitric oxide is a free radical, produced by most cells via Nitric Oxide (NO) synthase and plays stimulatory and inhibitory roles on cells. Physiological concentrations of NO are ess...

–SDS – Polyacrylamide Gel Electrophoresis (SDS PAGE) of blood plasma from three cattle and one buffalo affected with various tumors (odontoma, nasal granuloma and horn cancer in cattle and teat tumor in buffalo) revealed the presence of new proteins p51, p53 and p71-72 in all the samples which were not present in plasma from normal healthy bovines. However, another protein, p77.5 present in nor...

Seminal plasma is a promising biological fluid to use for noninvasive clinical diagnostics of male reproductive system disorders. To verify a list of prospective male infertility biomarkers, we developed a multiplex selected reaction monitoring assay and measured the relative abundance of 31 proteins in 30 seminal plasma samples from normal, nonobstructive azoospermia and post-vasectomy individ...

BACKGROUND Seminal plasma serves as a natural reservoir of antioxidants. It helps to remove excessive formation of reactive oxygen species (ROS) and consequently, reduce oxidative stress. Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and sperm dysfunction in infertile men. METHODS This prospective study consisted...

The human seminal plasma protein PSP94 is a small protein of 94 residues that contains ten cysteines. Since its discovery about 25 years ago, several potential biological functions have been reported for this protein. Many PSP94 homologues have also been identified since then from various species, but no crystal structure has been determined to date. PSP94 has been purified from human seminal p...