نتایج جستجو برای: cationic liposome

تعداد نتایج: 25602  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2000
N Shi W M Pardridge

Gene therapy of the brain is hindered by the presence of the blood-brain barrier (BBB), which prevents the brain uptake of bloodborne gene formulations. Exogenous genes have been expressed in the brain after invasive routes of administration, such as craniotomy or intracarotid arterial infusion of noxious agents causing BBB disruption. The present studies describe the expression of an exogenous...

Journal: :Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 2016

Journal: :The journal of gene medicine 2014
Chia-Ling Chan Kai K Ewert Ramsey N Majzoub Yeu-Kuang Hwu Keng S Liang Cecília Leal Cyrus R Safinya

BACKGROUND Cationic liposome (CL)-DNA complexes are promising gene delivery vectors with potential application in gene therapy. A key challenge in creating CL-DNA complexes for application is that their transfection efficiency (TE) is adversely affected by serum. In particular, little is known about the effects of a high serum content on TE, even though this may provide design guidelines for ap...

Journal: :Human gene therapy methods 2014
Salomé Magalhães Sofia Duarte Gabriel A Monteiro Fábio Fernandes

Nonviral vectors are highly attractive for gene therapy from a clinical point of view, and cationic lipid nanoparticles in particular have generated considerable interest. However, despite considerable recent advances, problems associated with low transfection efficiencies remain to be resolved to fully meet the potential of these vectors. The trafficking of plasmid DNA (pDNA) from the extracel...

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