While the addition of lime water to milk used as infant’s food has been common practice for many years, it has not been known just what changes the lime water produces, and as far 3s we know the subject has not been investigated from a purely chemical standpoint. The use of lime water in this connection has been based, first, upon the erroneous assumption that the acidity of COW’S milk is much ...

Expression levels of five mouse casein genes were analysed in the mammary gland of virgin, pregnant and lactating mice. We have already shown that the five murine casein genes are arranged in the order, alpha-beta-gamma-epsilon-kappa in a tandem array, very close to each other in a 250 kb DNA fragment of mouse genome. Northern blot analysis showed that, of the calcium-sensitive casein genes, th...

In this study, a gel/liquid interface is utilized for growing new (calcium-phosphate)/carrageenan garden. The hydrogels are made from carrageenan loaded with either sodium phosphate or calcium chloride, while the interfaced solution contains source of salt not used in (i. e. sodium-phosphate hydrogel calcium-chloride and vice versa). physical chemical properties tubes grown both systems same am...

The conjugation of polypeptides from a designed set to the small molecule ligand 3,5-bis[[bis(2-pyridylmethyl)amino]methyl]benzoic acid, which in the presence of Zn(2+) ions binds inorganic phosphate, has been shown to provide a polypeptide conjugate that binds α-casein, a multiply phosphorylated protein, with a dissociation constant K(D) of 17 nM. The measured affinity is more than three order...

A casein-type protein kinase has been isolated from cauliflower (Brassica cauliflora Gars.) nuclei and purified to a specific activity of 23,000 units/milligram of protein (1 unit is defined as the transfer of 1 picomole of (32)Pi from gamma-[(32)P]ATP to substrate per minute at 28 C). The enzyme has a molecular weight of approximately 39,000 as judged by sucrose density gradient sedimentation....

Cows’ milk contains inorganic calcium phosphate as a tightly-bound component of the casein micelles. The composition and chemical properties of this calcium phosphate have been studied for many years. Although the Ca/P ratio is similar to that found in hydroxyapatite, the use of other methods of characterization is desirable, since the various crystalline and amorphous forms of inorganic calciu...

To study the phosphorylation of one of the G-box binding factors from Arabidopsis (GBF1), we have obtained large amounts of this protein by expression in Escherichia coli. Bacterial GBF1 was shown to be phosphorylated very efficiently by nuclear extracts from broccoli. The phosphorylation activity was partially purified by chromatography on heparin-Sepharose and DEAE-cellulose and was character...

Zhang Y., Zhao X.-H. (2013): Properties of casein hydrolysate as affected by plastein reaction in ethanol-water medium. Czech J. Food Sci., 31: 559–567. Casein hydrolysate with in vitro ACE-inhibitory activity of 44.4% at 0.3 mg/ml was generated from casein by Alcalase and modified by the Alcalase-catalysed plastein reaction in an ethanol-water medium. Eight treated hydrolysates were prepared u...

BACKGROUND This study investigated the effect of three remineralizing agents on improving white spot lesions (WSLs). MATERIAL AND METHODS This clinical trial included children who had at least one WSL on anterior teeth of upper or lower jaws. The participants were randomly assigned to 4 groups by treatment: 1) a cream containing casein phosphopeptide-amorphous calcium phosphate and fluoride (...