BACKGROUND Carnitine has attracted scientific interest due to several health-related effects, like protection against neurodegeneration, mitochondrial decay, and oxidative stress as well as improvement of glucose tolerance and insulin sensitivity. The mechanisms underlying most of the health-related effects of carnitine are largely unknown. METHODS To gain insight into mechanisms through whic...

Butyrate is a potent anticarcinogenic compound against colon cancer cells in vitro. However, its rapid metabolism is hypothesized to limit its anticancer benefits in colonic epithelial cells. Carnitine, a potent antioxidant, is essential to fatty acid oxidation. The aims of this study were to identify a colon cancer cell line capable of transporting carnitine. We evaluated the effect of carniti...

BACKGROUND Functional carnitine deficiency [as indicated by an abnormal acyl-carnitine/free-carnitine (AC:FC) ratio] is commonly seen in patients with end-stage renal disease (ESRD), resulting in significant clinical detriments including anaemia, cardiomyopathy and muscle weakness. Nocturnal haemodialysis (NHD) (5-6 sessions per week, 8 h per treatment) has been reported to reverse several surr...

UNLABELLED In many eukaryotes, the carnitine shuttle plays a key role in intracellular transport of acyl moieties. Fatty acid-grown Saccharomyces cerevisiae cells employ this shuttle to translocate acetyl units into their mitochondria. Mechanistically, the carnitine shuttle should be reversible, but previous studies indicate that carnitine shuttle-mediated export of mitochondrial acetyl units t...

BACKGROUND L-carnitine is synthesized mainly in the liver and kidneys from lysine and methionine from dietary sources. Many reports have shown that L-carnitine can protect certain cells against the toxicity of several anticancer and toxic agents, although the detailed mechanism is poorly understood. In this study, we investigated the protective effect of L-carnitine and its molecular mechanism ...

This study was conducted to investigate developmental changes in the kinetics of carnitine palmitoyltransferase (CPT) within hepatic and skeletal muscle tissues of the canine species. Carnitine concentrations, CPT activity and the apparent K(m) for carnitine were measured in tissue homogenates from dogs in six age categories: newborn; 24-h-old; 3-, 6- and 9-wk-old; and adult. Hepatic CPT activi...

Four experiments were conducted to evaluate the effects of supplementing graded levels (0 to 100 ppm) of L-carnitine to the diet of weanling pigs on growth performance during a 34- to 38-d experimental period. A fifth experiment was conducted to determine the effects of addition of L-carnitine to diets with or without added soybean oil (SBO) on growth performance. In Exp. 1, 128 pigs (initial B...

Introduction: In order to clarify the characteristics of different administration routes and to optimize the dosage, carnitine kinetics was investigated under oral and intravenous supplementation. Methods: Lcarnitine was administered orally (900mg) for 12months, intravenously (1000 mg) for 3months and (500mg) for 3months sequentially in 18 chronic hemodialysis patients. Plasma free and acyl car...

The naturally occurring compatible solutes betaine and L-carnitine allow the food-borne pathogen Listeria monocytogenes to adjust to environments of high osmotic strength. Previously, it was demonstrated that L. monocytogenes possesses an ATP-dependent L-carnitine transporter (A. Verheul, F. M. Rombouts, R. R. Beumer, and T. Abee, J. Bacteriol. 177:3205-3212, 1995). The present study reveals th...