Carboxyhemoglobin levels in blood reflect endogenous carbon monoxide production and are often measured during routine blood gas analysis. Endogenous carbon monoxide production has been reported to be increased during sepsis, but carboxyhemoglobin levels have not been thoroughly evaluated as a biomarker of sepsis. We sought to determine whether carboxyhemoglobin levels were elevated during sepsi...

A simple, reliable and rapid method for the spectrophotometric determina­ tion of carboxyhemoglobin in fresh blood is described. A blood hemolysate is prepared by diluting a blood sample with 0.4 percent NH4 OH. Addition of sodium dithionite deoxygenates hemoglobin (and reduces methemoglobin if present) without affecting carboxyhemoglobin. Comparison of the 541/555 nm absorbance ratio for the h...

Erythrocytic 2,3-diphosphoglycerate (2,3-DPG), in response to carbon monoxide (CO) hypoxemia, was studied in 12 rabbits exposed to 250 ppm CO for 3 hours. Each animal served as its own control. Individual determinations of carboxyhemoglobin, arterial oxygen content, oxygen saturation, and 2,3-DPG were made before and immediately after exposures to both CO and normal air. After CO inhalation, th...

BACKGROUND Carbon monoxide (CO) is produced by reaction of isoflurane, enflurane, and desflurane in desiccated carbon dioxide absorbents. The inspiratory CO concentration depends on the dryness and identity of the absorbent and anesthetic. The adaptation of existing mathematical models to a rebreathing circuit allows identification of patient factors that predispose to more severe exposures, as...

This work presents the use of extended Modi ̄ed Lambert Beer (MLB) model for accurate and continuous monitoring of percent blood carboxyhemoglobin (COHb) (SCO) and oxyhemoglobin (OxyHb) saturation (SO2) via a ̄tting procedure. This quanti ̄cation technique is based on the absorption characteristics of hemoglobin derivatives in the wavelength range of 520–600 nm to give the best estimates of the re...

Methylene blue (MB) is frequently used as an antidote in treating methemoglobinemia (1) because it facilitates the reducing activity of the NADPH-dependent methemoglobin reductase system in erythrocytes (2). However, MB absorbs strongly between 550 and 700 nm (Fig. 1 ), the same spectrophotometric region as that of the various hemoglobin derivatives: oxyhemoglobin (O2Hb), deoxyhemoglobin (HHb),...

Amaç: Bu çalışmanın amacı acil servise başvuran akut karbon monoksit (CO) zehirlenmesinde yüksek karboksihemoglobin (CoHb) düzeyleri ile hiperbarik oksijen (HBO) tedavisi arasındaki ilişkiyi incelemektir.
 
 Gereç ve Yöntemler: CO zehirlenmesi nedeniyle değerlendirilen COHb düzeyi >20 olan hastalar retrospektif olarak incelendi. Hastaların aldıkları normobarik (NBO) ya da HBO tedav...

BACKGROUND A new eight-wavelength pulse oximeter is designed to measure methemoglobin and carboxyhemoglobin, in addition to the usual measurements of hemoglobin oxygen saturation and pulse rate. This study examines this device's ability to measure dyshemoglobins in human volunteers in whom controlled levels of methemoglobin and carboxyhemoglobin are induced. METHODS Ten volunteers breathed 50...

This study of 130 subjects was performed to obtain more data about the smoking habits and levels of carboxyhemoglobin in various types of pipe and cigar smokers. These smokers may be divided by history into primary or secondary smokers and also into current inhalers and noninhalers. Prior studies of carboxyhemoglobin levels have yielded conflicting results, presumably due to differences in curr...

Unlike ferrihemoglobin (Hb+), denatured carbonylhemoglobin (COHb) has almost the same absorption spectrum in the visible and near ultraviolet region as the native form; the very slight difference’appears only in the Soret band. Kinetic and equilibrium experiments under anaerobic conditions show that COHb is considerably more resistant to acid denaturation at 25’ than is Hb+ although the rates o...