objective(s): although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed.   materials and methods: single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. the isolate showed much greater endo-1, 4...

Substrate type is one of the major factors affecting growth and yield oyster mushroom (Pleurotus ostreatus). Five substrates; Finger millet husk, Fingermillet husk + Molasses, Rice bran, Wheat +Mustard oilseed cake, were evaluated for productivity mushroom. The experiment was conducted in a completely randomized design with each treatment replicated seven times. Additives added on finger @5% it...

We evaluated the effect of forage quality on response of cattle to supplementation with cooked molasses blocks. In Exp. 1, 175 heifers had ad libitum access to prairie hay (5.2% CP, DM basis). Treatments were a 2 x 3 factorial: supplementation with 0 or 1.96 kg/d of alfalfa DM, and supplementation with no cooked molasses block or with a low-protein or a high-protein cooked molasses block (14.4 ...

The lectin binding characteristics of goldfish retinal explants were examined by fluorescence microscopy. Neurites grown out from cultured retinal explants were found to bind concanavalin A, wheat germ agglutinin and ricin (agglutinin). The effects of tissue fixation on lectin binding to retinal explant neurites suggest that glycolipids may constitute the predominant ricin binding sites. A redu...

The purpose of the present study was follow the influence of periodical storage (at room temperature, 25-30°C), on the stability of oil including primary and secondary oxidation, total oxidation as well as conjugation. Color intensity at 430 nm of the oil samples was also evaluated to show changes of color during periodical storage. It was generally found peroxide value, panisidine, total oxida...

The influence of varying the amount of wheat germ agglutinin immobilized on Sepharose beads on the binding of glycoproteins to these beads was investigated. A series of wheat germ agglutinin-Sepharose gels containing between 0.10 and 10.0 mg of lectin/ml of gel was prepared, and the actual lectin content was established by acid hydrolysis of the gel followed by analysis of glycine, a major amin...

A metabolic trial was conducted at Buffalo Research Institute, Pattoki, in which 25 male buffalo calves were fed the diets having total mixed ration (100%TMR) (Tc), 70%TMR with 30% silage1 (T1), 70% TMR plus 30% silage2 (T2), 70%TMR with 30% silage3 (T3), and70% TMR plus 30% silage4 (T4). The data thus obtained was statistically analyzed using one way analysis of variance (ANOVA) technique. Amo...

BACKGROUND L-tryptophan is an important ingredient in medicines, especially in neuromedicines such as antidepressants. Many commercial processes employ various microorganisms with high tryptophan synthase activity to produce L-tryptophan from indole and L-serine, but these processes are very costly due to the costs of precursors, especially L-serine. OBJECTIVES For this reason, we studied the...

  Objective(s): Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed.   Materials and Methods: Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much ...

Proteolytic bacteria are most important for industries such as food and fermentation. Thus isolating and manipulating pure culture from various sources has importance for various biotechnology industries. In the present investigation a total of 26 bacteria were isolated from the dairy plant effluent. All isolates were screened for proteolytic activity using alkaline skim milk agar plate, among ...