Analyses of various lipid fractions of sections of cucumbers and of good and bloated dill pickles showed that marked changes occur in all lipid fractions during fermentation. The most striking difference noted was the decrease in the phospholipid fraction. A nearly fourfold increase in free fatty acid, as well as a marked increase in the neutral fat fatty acids and unsaponifiables, occurred. Ga...

We demonstrate the presence of an efficient, multispecific transport system for excretion of organic anions in the Malpighian tubules of the cricket Acheta domesticus using fluorescein (FL) as a model substrate. Malpighian tubules rapidly accumulated FL via a high affinity process (Km = 7.75 micromol l(-1)); uptake was completely eliminated by the prototypical organic anion transport inhibitor ...

The use of soaps as contact insecticides is of long standing in economic entomological practice, although there has apparently been no serious investigation leading toward the determination of the active principle chemically responsible for their value as such. References available to the writers attribute the toxicity of soaps to their alkaline ingredients, expressing the belief that their oth...

The antichlamydial effects of several fatty acids and monoglycerides were studied by incubating Chlamydia trachomatis bacteria with equal volumes of lipid solutions for 10 min and measuring the reduction in infectivity titer compared with that in a control solution without lipid. Caprylic acid (8:0), monocaprylin (8:0), monolaurin (12:0), myristic acid (14:0), palmitoleic acid (16:1), monopalmi...

Pharmacognosy Research,2023,15,1,71-74.DOI:10.5530/097484900265Published:December 2022Type:Original ArticleAuthors:Luciana Helena Veloso, Dario Alves de Oliveira, Cintya Neves Souza, Francine Souza Alves, Vany Ferraz, Anna Christina Almeida, Afrânio Farias Melo Júnior, Elytania Veiga Menezes, Murilo Malveira Brandão, and Vanessade Andrade Royo Author(s) affiliations:Luciana Veloso1, Oliveira1, ...

one of the most important aspects in recombinant biologic production, based on gmp rules, is the accuracy of final product quality control, especially assessment of host cell macromolecules contamination rate in final product. the purification requirement can be eliminated when the yeast cell containing the recombinant protein is used as a host cell. it is possibile that the final product conta...

Domoic acid (DA) is a potent toxin, marine biotoxin, and primarily produced by Pseudo-nitzschia. The DA hapten was coupled with bovine serum albumin (BSA), and ovalbumin (OVA) as carrier proteins. DA-BSA conjugate was used as immunogen and DA-OVA as coating antigen. Cell fusion between spleen cells and sp2/0 myeloma cells developed 1C3 hybridoma clone producing 1C3 monoclonal antibody (mAb). Hy...

The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH(3)(CH(2))(x)COOH, where x=6 (caprylic acid, CpA), 8 (capric acid, CA), 10 (lauric acid, LA), 12 (myristic acid, MA), 14 (palmitic acid, PA) and 16 (stearic acid, SA), is described. Control ESI-MS binding measurem...

The cell cycle proteins are key regulators of cell cycle progression whose deregulation is one of the causes of breast cancer. RNA interference (RNAi) is an endogenous mechanism to regulate gene expression and it could serve as the basis of regulating aberrant proteins including cell cycle proteins. Since the delivery of small interfering RNA (siRNA) is a main barrier for implementation of RNAi...