Serum succinate may offer an alternate analyte to lactate for the evaluation of hypoxia. To evaluate the potential uses of succinate, a relatively rapid capillary zone electrophoresis assay was developed for use in the clinical laboratory setting. Employing a simple indirect ultraviolet detection method with commercially available instrumentation, the limit of detection for serum succinate was ...

Soluble sugars play an important role in freezing tolerance in both herbaceous and woody plants, functioning in both the reduction of freezing-induced dehydration and the cryoprotection of cellular constituents. The quantification of soluble sugars in plant tissues is, therefore, essential in understanding freezing tolerance. While a number of analytical techniques and methods have been used to...

could not determine the CYP2B6 genotype of this individual because the PCR-RFLP assays failed to distinguish the two genotypes. An individual classified as CYP2B6*1/*7 or *5/*6 has also been reported by other authors (4). It is necessary to distinguish CYP2B6*1/*7 and *5/*6 because CYP2B6*5, *6, and *7 have been associated with changes in the production and function of CYP2B6, respectively (2, ...

A method for the determination of propionate in bread is described. The propionate was extracted from the bread with a repeated extraction procedure and measured using capillary zone electrophoresis in the indirect UV mode applying a background electrolyte of 0.005 M Tris adjusted at pH 4.6 by adding benzoic acid. Using laboratory-baked bread containing known amounts of sodium propionate, recov...

Capillary electrophoresis provides a new analytical tool that complements reversed-phase high-performance liquid chromatography separations of peptides. The principles of this technique and their application to developing peptide analyses are described. Detailed recipes for useful buffers and for preparation of the instrument are provided. A sequence of generally useful experiments is suggested...

The effect of order in separation media on DNA capillary zone electrophoresis is studied experimentally. A microfluidic technique based on colloidal self-assembly is employed to generate structures with different degrees of order. A model, SEM, and thermoporosimetry of the structures are employed to characterize media properties. The DNA mobility and dispersion coefficient is quantified in each...

Lisuride is an ergot alkaloid derivative with dopaminergic activity. It is used for treatment of Parkinsonism and some other diseases associated with high level of prolactine. Lisuride is a chiral compound derived from natural ergot alkaloids. A new capillary zone electrophoresis (CZE) method capable of separating the enantiomers of lisuride was developed. Using the optimized conditions (acidic...

Moniliformin is a mycotoxin produced by certain fungi pathogenic to maize. It is capable of causing disease in domestic animals, possibly through inhibition of pyruvate dehydrogenase. Testing for MON commonly involves extraction of maize, isolation of moniliformin using solid-phase extraction columns and detection with high-performance liquid chromatography (HPLC) or gas chromatography. A capil...

BACKGROUND Hemoglobin (Hb) A1c is widely used as the gold standard for blood glucose level monitoring in diabetic patients. Various methods are being used in clinical laboratories for HbA1c measurement. However, the presence of Hb variants may interfere with HbA1c measurement. METHODS The HbA1c assay was performed routinely in a 55-year old woman with type 2 diabetes and hyperlipidemia that w...