Background Agrobacterium mediated gene transfer technology offers the potential to introduce novel, high-value traits into selected, elite tree genotypes. Significant progress has been made in the regeneration and genetic transformation of Eucalyptus trees, but transformation efficiency has generally been low for eucalypts [1], especially for elite clonal genotypes. As a preliminary step toward...

We compared the growth of trees produced by micropropagation from nodal stem sections or callus tissue of a 20-year-old silver birch (Betula pendula Roth) tree with that of seedlings; growth was monitored for 17 months in pots followed by six years in the field. Micropropagated trees from both nodal stem sections and callus tissue grew at a similar rate to seedling trees and no obvious mutant t...

The effect of ifosfamide on bone healing was tested in a controlled experiment of fibular osteotomy in immature rabbits. Standardized shaft osteotomy was implemented in 10 experimental subjects (group 2) and 10 controls (group 1). Experimental animals received a 50 mg/kg ifosfamide dose by intraperitoneal injection on the fourth post-operative day, and for five days thereafter, while controls r...

Abstract Calotropis gigantea is a plant not widely used but has potential as medicinal plant. The phytochemical compound can be produced faster using tissue culture and combination of growth regulators. research objectives were to study the effect determine best concentration BAP 2,4-D in callus induction . This was carried out Plant Physiology Biotechnology Laboratory, Faculty Agriculture, Seb...

Abstract Callus formation and somatic embryogenesis in potato varieties are effective ways to obtain an entire plant from a single cell. The implementation of is widely used improving materials creating new biotechnological varieties. In our research, the optimum level 1-naphthaleneacetic acid (NAA) 6-benzyl aminopurine (BAP) was determined callus tissue local Sarnav variety, Desiree variety ta...

Most long-bone fractures heal through indirect or secondary fracture healing, a complex process in which endochondral ossification is an essential part and bone is regenerated by tissue differentiation. This process is sensitive to the mechanical environment, and several authors have proposed mechano-regulation algorithms to describe it using strain, pore pressure and/or interstitial fluid velo...

Stress fractures are overuse injuries of bone that affect elite athletes and military recruits. One response of cortical bone to stress fracture is to lay down periosteal callus. The objectives of this study were to determine if material properties are different among bones with different stages of stress fracture callus, at both a callus site and at a distal site. Cortical specimens were mecha...

Growth of an auxin-requiring soybean cotyledon callus tissue (Glycine max L., Merr. var. Acme) was promoted by tryptophan, tryptamine, indole, indoleacetamide and, to a very slight degree, anthranilic acid. When tryptophan-3-(14)C was supplied in the growth medium, labeled indoleacetic acid (IAA) was found in both the tissue and the medium. Medium, from which the cells had been removed, was als...

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...